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Genetics, Viruses, and Laboratory Techniques in Microbiology: Study Notes

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Central Dogma

  • Central Dogma
    05:45

Introduction to DNA Replication

  • Unwinding the DNA:Topoisomerase, Helicase & SSBs
    05:10
  • Components of DNA Replication
    04:47
  • Origin of Replication
    03:33

DNA Polymerases

  • DNA Polymerase Distinguishes Template from New Strand via Methylation
    03:47
  • DNA Polymerases Requirements
    05:03
  • DNA Polymerases
    04:01

Leading & Lagging DNA Strands

  • Leading & Lagging DNA Strands
    05:15

Steps of DNA Replication

  • Steps of DNA Replication
    09:29

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • Elongation of Transcription
    02:44
  • Initiation of Transcription
    04:16
  • Elongation of Transcription
    04:01

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Steps of Translation

  • Elongation of Translation
    04:59
  • Termination of Translation
    02:16
  • Steps of Translation
    05:03

Prokaryotic Gene Expression

  • Location of Transcription & Translation
    03:17
  • Simultaneous Transcription & Translation in Prokaryotes
    03:34
  • Prokaryotic Sigma (σ) Factors
    05:00

Review of Prokaryotic vs. Eukaryotic Gene Expression

  • Review of Prokaryotic vs. Eukaryotic Gene Expression
    05:30

Introduction to Regulation of Gene Expression

  • Positive vs Negative Gene Regulation
    02:17
  • Introduction to Regulation of Gene Expression
    06:40

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

Glucose's Impact on Lac Operon

  • Positive Control by cAMP & CRP
    08:32
  • Glucose's Impact on Lac Operon Example 1
    06:20
  • Glucose Levels, cAMP, & the Lac Operon
    05:58

The Trp Operon

  • In the Presence of Tryptophan
    03:11
  • The Trp Operon
    05:30
  • In the Absence of Tryptophan
    03:42

Review of the Lac Operon & Trp Operon

  • Review of the Lac Operon & Trp Operon
    06:24

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Methods of Inducing Mutations

  • Chemical Mutagens
    05:13
  • Radiation Mutagens
    02:55
  • Methods of Inducing Mutations
    03:19

The Ames Test

  • The Ames Test
    06:59

Introduction to DNA Repair

  • Introduction to DNA Repair
    02:47

DNA Repair Mechanisms

  • Nucleotide Excision Repair
    03:31
  • SOS Repair System
    03:50
  • Photoreactivation
    02:29

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

Conjugation: F Plasmids

  • F Plasmids
    05:57
  • Mechanism of F Plasmid Conjugation in E. coli
    04:12

Conjugation: Hfr & F' Cells

  • F’ Cell Conjugation
    04:31
  • Hfr Cell Conjugation
    09:41

Genome Variability

  • Phage DNA
    01:44
  • Genome Variability
    01:59
  • Genome Variability
    03:18

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Introduction to Viruses

  • General Structure of Viruses
    04:47
  • Three Common Shapes of Viruses
    03:19
  • Enveloped vs. Non-Enveloped Viruses
    05:09

Introduction to Bacteriophage Infections

  • Map of Lesson on Bacteriophage Infections
    03:52
  • Productive vs. Latent State Infections
    04:09
  • Lytic & Lysogenic Bacteriophages
    04:34

Bacteriophage: Lytic Phage Infections

  • Bacteriophage: Lytic Phage Infections
    04:58

Bacteriophage: Lysogenic Phage Infections

  • Bacteriophage: Lysogenic Phage Infections
    05:27
  • Lysogenic Conversion
    03:37

Plaque Assays

  • Plaque Assays
    04:21

Introduction to Animal Virus Infections

  • Introduction to Animal Virus Infections
    02:33
  • Memorizing the Steps of Animal Virus Infections
    01:40

Animal Viruses: 1. Attachment to the Host Cell

  • Animal Viruses: 1. Attachment to the Host Cell
    03:12

Animal Viruses: 2. Entry & Uncoating in the Host Cell

  • Entry & Uncoating by Endocytosis
    02:29
  • Animal Viruses: 2. Entry & Uncoating in the Host Cell
    04:29
  • Entry & Uncoating of Enveloped Viruses by Membrane Fusion
    02:59

Animal Viruses: 3. Synthesis & Replication

  • Map of Lesson on Animal Virus Synthesis & Replication
    03:34
  • Types of RNA Virus Genomes
    10:46
  • Common Themes of All Virus Genomes
    02:58

Animal Viruses: DNA Virus Synthesis & Replication

  • Animal Viruses: DNA Virus Synthesis & Replication
    01:10
  • Replication of Single-Stranded DNA (ssDNA) Viruses
    04:12
  • Replication of Double-Stranded DNA (dsDNA) Viruses
    03:58

Animal Viruses: RNA Virus Synthesis & Replication

  • Animal Viruses: RNA Virus Synthesis & Replication
    01:30
  • Replication of (+) Single Strand RNA (ssRNA) Viruses
    03:52
  • Replication of Double-Stranded RNA Viruses
    04:28

Animal Viruses: Antigenic Drift vs. Antigenic Shift

  • Animal Viruses: Antigenic Drift vs. Antigenic Shift
    05:27

Animal Viruses: 4. Assembly Inside Host Cell

  • Animal Viruses: 4. Assembly Inside Host Cell
    04:26

Animal Viruses: 5. Release from Host Cell

  • Release of Non-Enveloped Viruses
    02:34
  • Release of Enveloped Viruses
    05:08

Prions

  • Prion Development in Neurons Leads to Scrapie
    04:00
  • Prions
    03:05

Simple Staining

  • Simple Staining
    03:23
  • Simple Staining Basic & Acidic Dyes
    05:30

Differential Staining

  • Differential Staining
    04:07

Gram Stain

  • Gram Stain
    04:57

Bacterial Cell Morphology & Arrangements

  • Types of Bacterial Cell Morphology
    02:44
  • Bacterial Cell Morphology & Arrangements
    01:13
  • Bacilli Bacterial Cell Arrangements
    03:56