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Microbial Genetics: The Central Dogma, Gene Expression, and Genetic Variation

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Central Dogma

  • Central Dogma
    05:45

Introduction to DNA Replication

  • Unwinding the DNA:Topoisomerase, Helicase & SSBs
    05:10
  • Components of DNA Replication
    04:47
  • Origin of Replication
    03:33

DNA Polymerases

  • DNA Polymerase Distinguishes Template from New Strand via Methylation
    03:47
  • DNA Polymerases Requirements
    05:03
  • DNA Polymerases
    04:01

Leading & Lagging DNA Strands

  • Leading & Lagging DNA Strands
    05:15

Steps of DNA Replication

  • Steps of DNA Replication
    09:29

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • Elongation of Transcription
    02:44
  • Initiation of Transcription
    04:16
  • Elongation of Transcription
    04:01

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Steps of Translation

  • Elongation of Translation
    04:59
  • Termination of Translation
    02:16
  • Steps of Translation
    05:03

Introduction to Regulation of Gene Expression

  • Positive vs Negative Gene Regulation
    02:17
  • Introduction to Regulation of Gene Expression
    06:40

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

Glucose's Impact on Lac Operon

  • Positive Control by cAMP & CRP
    08:32
  • Glucose's Impact on Lac Operon Example 1
    06:20
  • Glucose Levels, cAMP, & the Lac Operon
    05:58

The Trp Operon

  • In the Presence of Tryptophan
    03:11
  • The Trp Operon
    05:30
  • In the Absence of Tryptophan
    03:42

Review of the Lac Operon & Trp Operon

  • Review of the Lac Operon & Trp Operon
    06:24

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Methods of Inducing Mutations

  • Chemical Mutagens
    05:13
  • Radiation Mutagens
    02:55
  • Methods of Inducing Mutations
    03:19

Introduction to DNA Repair

  • Introduction to DNA Repair
    02:47

DNA Repair Mechanisms

  • Nucleotide Excision Repair
    03:31
  • SOS Repair System
    03:50
  • Photoreactivation
    02:29

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

Genome Variability

  • Phage DNA
    01:44
  • Genome Variability
    01:59
  • Genome Variability
    03:18