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Microbial Metabolism, Growth, Genetics, Control, and Antimicrobial Agents: Study Guide for BIOL 2420 Exam #2

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Introduction to Energy

ATP

Enzymes

Enzyme Activation Energy

Enzyme Inhibition

Redox Reactions

Introduction to Aerobic Cellular Respiration

Glycolysis

Pyruvate Oxidation

Krebs Cycle

Electron Transport Chain

Chemiosmosis

Fermentation & Anaerobic Respiration

Binary Fission

Generation Times

Introduction to Cultivating Microbial Growth

Types of Solid Culture Media

Selective Media

Differential Media

Biofilms

Microbial Growth Curves in a Closed System

Temperature Requirements for Microbial Growth

Oxygen Requirements for Microbial Growth

pH Requirements for Microbial Growth

Osmolarity Factors for Microbial Growth

Nutritional Factors of Microbial Growth

The Ames Test

Introduction to Mutations

Horizontal Gene Transfer

Bacterial Transformation

Transduction

Introduction to Conjugation

Conjugation: F Plasmids

Central Dogma

Introduction to DNA Replication

Steps of DNA Replication

Introduction to Transcription

Steps of Transcription

Introduction to Translation

Steps of Translation

Prokaryotic Gene Regulation via Operons

The Lac Operon

Introduction to DNA-Based Technology

Introduction to DNA Cloning

Steps to DNA Cloning

Introduction to Polymerase Chain Reaction

The Steps of PCR

Gel Electrophoresis

The Griffith Experiment