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Microbial Pathogenicity, Disease Principles, Biotechnology, and Microbial Genetics: Study Notes

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Bacterial Pathogenesis

  • Intro to Bacterial Pathogenesis
    02:35
  • 1) Exposure to Pathogen
    03:27
  • 3) Invasion into Host Tissues
    05:38

Introduction to Avoiding Host Defense Mechanisms

  • Introduction to Avoiding Host Defense Mechanisms
    01:59

The Glycocalyx: Capsules & Slime Layers

  • The Glycocalyx: Capsules & Slime Layers
    02:26
  • Capsules vs. Slime Layers
    01:53

Introduction to Biofilms

  • Introduction to Biofilms
    03:31

Introduction to Immunity

  • Innate vs. Adaptive Immunity
    05:52
  • Antigens & Antibodies
    02:58

Introduction to Epidemiology

  • Introduction to Epidemiology Example 1
    01:48
  • Introduction to Epidemiology Example 2
    05:15
  • Mortality & Case-Fatality Rates
    02:28

Healthcare-Associated Infections

  • Risk Factors Contributing to HAIs
    02:45
  • Healthcare-Associated Infections
    01:33
  • Preventing HAIs
    01:59

Emerging Infectious Diseases

  • Emerging Infectious Diseases Example 1
    02:11
  • Factors Contributing to Emerging & Re-Emerging Infectious Diseases
    03:21
  • Emerging & Re-Emerging Infectious Diseases
    03:15

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Introduction to DNA-Based Technology

  • Map of DNA-Based Technology Lesson
    03:12
  • Introduction to DNA-Based Technology
    02:30

Introduction to DNA Cloning

  • Introduction to DNA Cloning
    02:25
  • Cloning with Recombinant DNA
    03:41

Steps to DNA Cloning

  • Steps to DNA Cloning
    04:42
  • 1b) Use Ligation Enzymes
    04:58
  • 1a) Use Restriction Enzymes
    03:27

Introduction to Polymerase Chain Reaction

  • Introduction to Polymerase Chain Reaction
    01:31
  • Components of a Polymerase Chain Reaction
    05:57
  • Why do we use PCR?
    02:53

The Steps of PCR

  • 1) Denaturation
    03:33
  • 2) Annealing
    02:56
  • The Steps of PCR
    04:39

DNA Fingerprinting

  • Short Tandem Repeats (STRs)
    03:41
  • DNA Fingerprinting
    03:30

Southern Blotting

  • Southern Blotting
    03:31
  • Steps of Southern Blotting
    08:21

CRISPR CAS

  • CRISPR CAS
    07:43

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Introduction to Regulation of Gene Expression

  • Positive vs Negative Gene Regulation
    02:17
  • Introduction to Regulation of Gene Expression
    06:40

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

Glucose's Impact on Lac Operon

  • Positive Control by cAMP & CRP
    08:32
  • Glucose's Impact on Lac Operon Example 1
    06:20
  • Glucose Levels, cAMP, & the Lac Operon
    05:58

The Trp Operon

  • In the Presence of Tryptophan
    03:11
  • The Trp Operon
    05:30
  • In the Absence of Tryptophan
    03:42

Review of the Lac Operon & Trp Operon

  • Review of the Lac Operon & Trp Operon
    06:24

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Methods of Inducing Mutations

  • Chemical Mutagens
    05:13
  • Radiation Mutagens
    02:55
  • Methods of Inducing Mutations
    03:19

Mutant Detection

  • Mutant Detection
    06:49
  • Replica Plating
    05:36

The Ames Test

  • The Ames Test
    06:59

Introduction to DNA Repair

  • Introduction to DNA Repair
    02:47

DNA Repair Mechanisms

  • Nucleotide Excision Repair
    03:31
  • SOS Repair System
    03:50
  • Photoreactivation
    02:29

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

Conjugation: F Plasmids

  • F Plasmids
    05:57
  • Mechanism of F Plasmid Conjugation in E. coli
    04:12

Conjugation: Hfr & F' Cells

  • F’ Cell Conjugation
    04:31
  • Hfr Cell Conjugation
    09:41