Question 1

Which biosafety level is appropriate for handling Bacillus subtilis, and what is the primary reason for this classification?

Accepted Answer

BSL-1, because it generally does not cause disease in healthy humans.

Question 2

A laboratory is working with Ebola virus. Which biosafety level must be used, and what are two key facility requirements for this level?

Accepted Answer

BSL-4; requires isolated facilities and decontamination showers for personnel.

Question 3

Which agency is primarily responsible for regulating occupational safety and health standards in microbiological laboratories in the United States?

Accepted Answer

OSHA (Occupational Safety and Health Administration)

Question 4

Which organism would require a laboratory to operate at biosafety level 3 due to its potential to cause severe disease or lethality?

Accepted Answer

Bacillus anthracis

Question 5

A laboratory worker is exposed to a chemical that causes severe tissue injury. According to the NIOSH Pocket Guide, which section of the Material Safety Data Sheet (MSDS) should they consult first?

Accepted Answer

Section 4: First Aid Measures

Question 6

Which fire extinguisher type is suitable for electrical fires, and what symbol is used to identify it?

Accepted Answer

Type C, blue circle

Question 7

A laboratory technician is working with Salmonella spp. Which biosafety level is required, and what additional safety measures must be implemented compared to BSL-1?

Accepted Answer

BSL-2; containment devices and stricter guidelines must be implemented.

Question 8

Which piece of laboratory equipment is essential for sterilizing media and instruments using high-pressure steam, and what is the typical temperature used?

Accepted Answer

Autoclave; $$121^\circ C$$

Question 9

A laboratory worker is required to wear a dosimeter badge. What hazard is this badge designed to monitor?

Accepted Answer

Radiation exposure

Question 10

Which biosafety level requires personnel to take decontamination showers each time they leave the laboratory, and why is this necessary?

Accepted Answer

BSL-4; to prevent life-threatening infections from dangerous exotic agents.

Question 11

Which organism listed below is NOT appropriate for handling in a BSL-1 laboratory?

Accepted Answer

Staphylococcus aureus

Question 12

A laboratory is disposing of broken glassware. Which container should be used for this waste, and why?

Accepted Answer

Glass disposal box; because it is specifically designed for non-infectious glass waste.

Question 13

Which protective gear is mandatory in many laboratories to prevent eye injuries, and why are regular glasses not sufficient?

Accepted Answer

Eye protection; because regular glasses do not provide adequate coverage against splashes.

Question 14

A laboratory is handling hepatitis B virus. Which biosafety level is required, and what is the rationale for this classification?

Accepted Answer

BSL-2; because it is a moderate risk agent that can cause disease but is not highly lethal.

Question 15

Which biosafety cabinet is used to prevent or minimize exposure to biohazardous agents, and what is the primary mechanism by which it protects laboratory workers?

Accepted Answer

Biosafety cabinet; by using HEPA-filtered airflow to contain biohazardous agents.

Question 16

A laboratory worker is exposed to repeated small doses of ionizing radiation. What is the most likely long-term health effect, and which organ system is most at risk?

Accepted Answer

Radiation sickness; bone marrow and central nervous system

Question 17

Which biosafety level is required for handling Marburg virus, and what is the primary risk associated with this agent?

Accepted Answer

BSL-4; high risk of aerosol transmission and life-threatening infection

Question 18

A laboratory is required to keep updated records of all containment guidelines and life support systems. Which biosafety level mandates this daily inspection and record-keeping?

Accepted Answer

BSL-4

Question 19

Which agency introduced the concept of 'Risk Groups' for classifying biological agents, and how many groups are there?

Accepted Answer

NIH; 4 groups

Chapter 9

Study Guide - Practice Questions

Study Guide - Flashcards