Question 1

Which of the following best describes the process and significance of Koch’s postulates in microbiology?

Accepted Answer

They are a set of criteria used to determine if a specific microorganism causes a specific disease, involving isolation, cultivation in pure culture, and reproduction of disease in a healthy host.

Question 2

A scientist wants to isolate a single species of bacteria from a mixed sample. Which technique should they use and why is it important?

Accepted Answer

Streaking the sample on a solid medium to obtain a pure culture, which is essential for studying the characteristics of a single species without interference from others.

Question 3

Which of the following statements correctly distinguishes between a cell wall and a cytoplasmic membrane?

Accepted Answer

The cell wall provides structural support and shape, while the cytoplasmic membrane controls the movement of substances into and out of the cell.

Question 4

Which of the following best explains why cyanobacteria were crucial in the evolution of life on Earth?

Accepted Answer

They performed oxygenic photosynthesis, releasing $O_2$ into the atmosphere and enabling the evolution of aerobic organisms.

Question 5

A Gram-negative bacterium is subjected to the Gram stain procedure. What color will it appear under the microscope, and why?

Accepted Answer

Pink/red, because the thin peptidoglycan layer does not retain the crystal violet-iodine complex after decolorization.

Question 6

Which of the following is a major advantage of phase-contrast microscopy over traditional staining techniques?

Accepted Answer

It allows observation of living cells without killing them, as staining often does.

Question 7

Which of the following best describes the difference between magnification and resolution in microscopy?

Accepted Answer

Magnification increases the apparent size of an object, while resolution is the ability to distinguish two close objects as separate.

Question 8

Which of the following statements about the three domains of life is correct?

Accepted Answer

Eukarya is the only domain that contains eukaryotic organisms; Bacteria and Archaea are both prokaryotic.

Question 9

Which of the following best explains why Archaea are more closely related to Eukarya than to Bacteria?

Accepted Answer

Archaea and Eukarya share more similarities in their ribosomal RNA sequences and certain aspects of their molecular biology.

Question 10

A researcher is using an electron microscope to study a bacterial cell. Which type of electron microscope should they use to view the internal structures, and why?

Accepted Answer

Transmission electron microscope (TEM), because it allows visualization of internal cell structures at high resolution.

Question 11

Which of the following best describes the process of horizontal gene transfer in microorganisms?

Accepted Answer

The movement of genetic material between organisms other than by vertical transmission from parent to offspring.

Question 12

Which of the following is a correct statement about the function of ribosomes in microbial cells?

Accepted Answer

Ribosomes are the sites of protein synthesis, where translation of mRNA into polypeptides occurs.

Question 13

Which of the following best explains how Pasteur disproved the theory of spontaneous generation?

Accepted Answer

He used swan-necked flasks to show that microorganisms did not grow in sterilized broth unless exposed to air, demonstrating that life does not arise spontaneously.

Question 14

A patient is diagnosed with a bacterial infection. According to Koch’s postulates, which step must be completed to confirm the causative agent?

Accepted Answer

The suspected pathogen must be isolated from the diseased host and grown in pure culture.

Question 15

Which of the following best describes the role of the gut microbiome in human nutrition?

Accepted Answer

It helps digest complex carbohydrates and synthesizes certain vitamins, contributing to overall health.

Question 16

Which of the following is an example of a macromolecule found in microbial cells?

Accepted Answer

Polysaccharide

Question 17

Which of the following best describes the function of staining in light microscopy?

Accepted Answer

It increases contrast between cells and their background, making cellular structures more visible.

Question 18

Which of the following is a correct statement about enrichment culture techniques?

Accepted Answer

They use specific growth conditions to favor the growth of particular microorganisms from a mixed sample.

Question 19

Which of the following is a correct definition of a phylogenetic tree?

Accepted Answer

A diagram that shows the evolutionary relationships among different organisms based on genetic or physical characteristics.

Question 20

Which of the following is a correct method for sterilizing microbiological media?

Accepted Answer

Autoclaving at $121^\circ C$ and 15 psi for 15 minutes.

Microbiology Chapter 1 Study Guide – Key Concepts and Step-by-Step Guidance

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