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Microbiology Core Concepts: Learning Objectives and Study Guide

Study Guide - Learn with Videos

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Introduction to Spontaneous Generation

Francesco Redi's Experiments

Needham vs. Spallanzani

Pasteur's Experiments on Spontaneous Generation

John Tyndall's Experiment

History of Spontaneous Generation Summarized

Introduction to Microscopes

Magnification, Resolution, & Contrast

Introduction to Light Microscopy

Light Microscopy: Bright-Field Microscopes

Light Microscopes that Increase Contrast

Light Microscopes that Detect Fluorescence

Electron Microscopes

Reviewing the Different Types of Microscopes

Introduction to Staining

Simple Staining

Differential Staining

Other Types of Staining

Reviewing the Types of Staining

Gram Stain

Introduction to Bacterial Cell Walls

Gram-Positive Cell Walls

Gram-Negative Cell Walls

Gram-Positive vs. Gram-Negative Cell Walls

Bacterial Cell Morphology & Arrangements

Introduction to Prokaryotic Flagella

Prokaryotic Flagellar Structure

Prokaryotic Flagellar Movement

Proton Motive Force Drives Flagellar Motility

Chemotaxis

Review of Prokaryotic Surface Structures

Binary Fission

Generation Times

Introduction to Cultivating Microbial Growth

Growing a Pure Culture

Microbial Growth Curves in a Closed System

Temperature Requirements for Microbial Growth

Osmolarity Factors for Microbial Growth

Oxygen Requirements for Microbial Growth

pH Requirements for Microbial Growth

Measuring Growth by Direct Cell Counts

Measuring Growth by Plate Counts

Measuring Growth by Membrane Filtration

Measuring Growth by Biomass

Introduction to the Types of Culture Media

Types of Solid Culture Media

Selective Media

Differential Media

Reducing Media

Enrichment Media

Reviewing the Types of Culture Media

Introduction to Controlling Microbial Growth

Selecting a Method to Control Microbial Growth

Physical Methods to Control Microbial Growth

Chemical Methods to Control Microbial Growth

Chemicals Used to Control Microbial Growth

Liquid Chemicals: Alcohols, Aldehydes, & Biguanides

Liquid Chemicals: Halogens

Liquid Chemicals: Surface-Active Agents

Chemical Gases: Ethylene Oxide, Ozone, & Formaldehyde

Review of Chemicals Used to Control Microbial Growth

Chemical Preservation of Perishable Products

Introduction to Microbial Genetics

Introduction to Mutations

Methods of Inducing Mutations

Mutant Detection

The Ames Test

Introduction to DNA Repair

DNA Repair Mechanisms

Horizontal Gene Transfer

Bacterial Transformation

Transduction

Introduction to Conjugation

Conjugation: F Plasmids

Conjugation: Hfr & F' Cells