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Microbiology Course Learning Objectives and Key Concepts

Study Guide - Learn with Videos

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Introduction to Microbiology

Introduction to Taxonomy

Scientific Naming of Organisms

Members of the Bacterial World

Introduction to Bacteria

Introduction to Archaea

Introduction to Eukarya

Scientific Method

Experimental Design

Introduction to Spontaneous Generation

Francesco Redi's Experiments

Needham vs. Spallanzani

Pasteur's Experiments on Spontaneous Generation

John Tyndall's Experiment

History of Spontaneous Generation Summarized

Introduction to Microscopes

Magnification, Resolution, & Contrast

Introduction to Light Microscopy

Light Microscopy: Bright-Field Microscopes

Light Microscopes that Increase Contrast

Light Microscopes that Detect Fluorescence

Electron Microscopes

Reviewing the Different Types of Microscopes

Introduction to Staining

Simple Staining

Differential Staining

Other Types of Staining

Gram Stain

Introduction to Cultivating Microbial Growth

Growing a Pure Culture

Types of Solid Culture Media

Plating Methods

Introduction to the Types of Culture Media

Chemically Defined Media

Complex Media

Selective Media

Differential Media

Introduction to Biomolecules

Monomers & Polymers

Carbohydrates

Proteins

Nucleic Acids

Lipids

Cell Envelope & Biological Membranes

Bacterial & Eukaryotic Cell Membranes

Archaeal Cell Membranes

Introduction to Membrane Transport

Passive vs. Active Transport

Osmosis

Simple and Facilitated Diffusion

Active Transport

Binary Fission

Generation Times

Bacterial Cell Morphology & Arrangements

Overview of Prokaryotic Cell Structure

Introduction to Bacterial Cell Walls

Gram-Positive Cell Walls

Gram-Negative Cell Walls

Gram-Positive vs. Gram-Negative Cell Walls

The Glycocalyx: Capsules & Slime Layers

Introduction to Biofilms

Pili

Fimbriae & Hami

Introduction to Prokaryotic Flagella

Prokaryotic Flagellar Structure

Prokaryotic Flagellar Movement

Chemotaxis

Prokaryotic Ribosomes

Introduction to Bacterial Plasmids

Cell Inclusions

Endospores

Sporulation

Germination

Mitosis & Meiosis

Introduction to Eukaryotic Organelles

Endomembrane System: Protein Secretion

Endomembrane System: Digestive Organelles

Mitochondria & Chloroplasts

Endosymbiotic Theory

Introduction to the Cytoskeleton

Eukaryotic Cilia & Flagella

The Griffith Experiment

The Hershey-Chase Experiment

Chargaff's Rules

Discovering the Structure of DNA

Meselson-Stahl Experiment

Introduction to DNA Replication

DNA Polymerases

Leading & Lagging DNA Strands

Steps of DNA Replication

DNA Repair

Central Dogma

Introduction to Transcription

Steps of Transcription

Transcription Termination in Prokaryotes

Eukaryotic RNA Processing and Splicing

Introduction to Types of RNA

Genetic Code

Introduction to Translation

Steps of Translation

Review of Transcription vs. Translation

Prokaryotic Gene Expression

Review of Prokaryotic vs. Eukaryotic Gene Expression

Introduction to Regulation of Gene Expression

Prokaryotic Gene Regulation via Operons

The Lac Operon

Glucose's Impact on Lac Operon

The Trp Operon

Review of the Lac Operon & Trp Operon

Introduction to Eukaryotic Gene Regulation

Eukaryotic Chromatin Modifications

Eukaryotic Transcriptional Control

Eukaryotic Post-Transcriptional Regulation

Post-Translational Modification

Eukaryotic Post-Translational Regulation

Introduction to Microbial Genetics

Introduction to Mutations

Methods of Inducing Mutations

Prototrophs vs. Auxotrophs

Mutant Detection

The Ames Test

Introduction to DNA Repair

DNA Repair Mechanisms

Horizontal Gene Transfer

Bacterial Transformation

Transduction

Introduction to Conjugation

Conjugation: F Plasmids

Conjugation: Hfr & F' Cells

Genome Variability

CRISPR CAS

Introduction to Viruses

Introduction to Bacteriophage Infections

Bacteriophage: Lytic Phage Infections

Bacteriophage: Lysogenic Phage Infections

Bacteriophage: Filamentous Phage Infections

Plaque Assays

Introduction to Animal Virus Infections

Animal Viruses: 1. Attachment to the Host Cell

Animal Viruses: 2. Entry & Uncoating in the Host Cell

Animal Viruses: 3. Synthesis & Replication

Animal Viruses: DNA Virus Synthesis & Replication

Animal Viruses: RNA Virus Synthesis & Replication

Animal Viruses: Antigenic Drift vs. Antigenic Shift

Animal Viruses: Reverse-Transcribing Virus Synthesis & Replication

Animal Viruses: 4. Assembly Inside Host Cell

Animal Viruses: 5. Release from Host Cell

Acute vs. Persistent Viral Infections

Viroids

Prions

Biofilms