Question 1

Which of the following is NOT allowed in the microbiology lab?

Accepted Answer

Eating or drinking

Question 2

Before leaving the lab, you should always:

Accepted Answer

Wash your hands and disinfect your workspace

Question 3

The main goal of lab safety rules is to:

Accepted Answer

Reduce risk

Question 4

Magnification refers to:

Accepted Answer

The increase of image size in specimen view

Question 5

Resolution is defined as:

Accepted Answer

The ability to distinguish two points as separate

Question 6

The limit of resolution formula is:

Accepted Answer

$d = \lambda / (2 	imes NA)$

Question 7

As magnification increases, the field of view (FOV):

Accepted Answer

Decreases

Question 8

The depth of field (DOF):

Accepted Answer

Becomes shallower with higher magnification

Question 9

Contrast in microscopy is improved by:

Accepted Answer

Staining the specimen

Question 10

A cluster of spherical cells is a:

Accepted Answer

Staphylococcus

Question 11

Rod-shaped bacteria are known as:

Accepted Answer

Bacilli

Question 12

Differential staining helps to:

Accepted Answer

Differentiate between cell types or structures

Question 13

In a basic dye, the chromophore carries a:

Accepted Answer

Positive charge

Question 14

After decolorization, Gram-negative bacteria appear:

Accepted Answer

Colorless

Question 15

The primary stain in the acid-fast stain is:

Accepted Answer

Carbol fuchsin

Question 16

The acid-fast stain identifies bacteria with:

Accepted Answer

Waxy lipid cell walls

Question 17

Aseptic technique aims to:

Accepted Answer

Prevent contamination of cultures and environment

Question 18

UV light damages DNA by forming:

Accepted Answer

Thymine dimers

Question 19

A pure culture contains:

Accepted Answer

A single microbial species

Question 20

A psychrophile grows best at:

Accepted Answer

0-20 $^\circ$C

Microbiology Lab: Key Concepts and Techniques

Study Guide - Practice Questions