Ch 13 Viruses, Viroids, and Prions: Structure, Multiplication, and Pathogenicity

Viruses, Viroids, and Prions

Characteristics and Structure of Viruses

Viruses are unique infectious agents that differ fundamentally from bacteria and other microorganisms. They are obligate intracellular parasites, meaning they require a living host cell to replicate.

• Size: Viruses are extremely small, ranging from 20 to 300 nm, and are filterable.

• Nucleic Acid: Each virus contains either DNA or RNA, never both.

• Capsid: The nucleic acid is surrounded by a protein coat called the capsid, which is made up of subunits called capsomeres.

• Envelope: Some viruses have an envelope composed of lipids, carbohydrates, and proteins; those without are termed "naked" or non-enveloped viruses.

• Virion: The complete, infectious viral particle is called a virion.

• Spikes: Viral envelopes may have spikes that aid in attachment and can be associated with virulence.

• Metabolism: Viruses lack metabolic enzymes and are inert outside the host cell.

Viral Morphology

Viral morphology is determined by the structure of the capsid and can be classified into four main types:

• Helical: Capsids are hollow cylinders with nucleic acid inside. Examples include Tobacco mosaic virus and Ebola virus.

• Polyhedral: Many-sided, usually icosahedral (20 sides). Examples include Adenovirus and Poliovirus.

• Enveloped: Viruses are covered by an envelope and are often roughly spherical but pleomorphic. Examples include Influenza virus (enveloped helical) and Herpes simplex virus (enveloped polyhedral).

• Complex: These have complicated structures, often seen in bacteriophages, with polyhedral heads and helical tails.

Host Range and Viral Attachment

The host range of a virus is determined by its ability to attach to specific receptor sites on the host cell and the availability of host cell factors required for replication. All living cells can be infected by viruses, but each virus has a specific host range.

Isolation and Cultivation of Viruses

Viruses require living hosts for growth and cultivation in the laboratory.

• Animal hosts: Some viruses can only be cultured in animals (e.g., HIV).

• Embryonated eggs: Used especially for vaccine production; viral growth may result in embryo death.

• Cell culture: The preferred method, where cells form a monolayer in a dish containing growth media.

Cytopathic Effects of Viral Infection

Viral infection can cause visible changes in host cells, known as cytopathic effects (CPE).

• Cell death

• Inclusion bodies: e.g., Negri bodies in rabies

• Giant cells: Host cell fusion

• Interferon production: Protective to nearby uninfected cells; potential anticancer agent

Identification of Viruses

Virus identification is challenging and involves several methods:

• Electron microscopy

• Cytopathic effects

• Serological tests: Most common, including viral hemagglutination and neutralization tests

• PCR: Used to amplify viral nucleic acid

Viral Multiplication

Viral multiplication requires invasion of the host cell and hijacking of its metabolic machinery.

• Viral genes

• Capsid proteins

• Viral enzymes

• Host enzymes: Ribosomes, tRNA, ATP production

Multiplication of Bacteriophages

Bacteriophages multiply by two mechanisms:

1. Lytic cycle: Ends with death of the host cell. Steps:

   1. Attachment

   2. Penetration

   3. Biosynthesis

   4. Maturation

   5. Lysis

2. Lysogeny: Host cell remains alive. Steps:

   1. Attachment

   2. Penetration

   3. Phage nucleic acid recombines with host chromosome (prophage)

   4. Prophage is replicated with chromosome

   5. Prophages may confer new properties to host

   6. Prophages may be induced into the lytic cycle

Multiplication of Animal Viruses

Animal viruses multiply similarly to phages but with key differences:

1. Attachment: Mediated by spikes, fibers, and host cell receptors

2. Entry: Occurs by pinocytosis or membrane fusion

3. Uncoating and biosynthesis: DNA viruses replicate in the nucleus; capsid and other structures are made in the cytoplasm. RNA viruses may convert RNA to DNA (e.g., HIV).

4. Maturation and release: May involve budding (enveloped viruses) or cell lysis (naked viruses)

Viruses and Cancer

Some viruses can cause cancer by altering the host cell's DNA.

• ~10% of cancers are virally induced

• Oncogenes: Genes that can cause cancerous transformation when mutated

• Contact inhibition: Loss of this property leads to uncontrolled cell growth

Latent and Persistent Viral Infections

Viruses may remain dormant (latent) or continuously released (persistent) in host cells.

• Latent infections: Virus remains in host cells for extended periods without symptoms (e.g., Herpes simplex, Varicella zoster)

• Persistent infections: Virus is continuously released, causing chronic effects (e.g., HIV, Hepatitis B)

Disease	Primary Effect	Causative Virus
Cold sores	No symptoms during latency, viruses not usually released	Herpes simplex 1 and 2
Leukemia	Skin and mucous membrane lesions; genital lesions	HTLV-1 and 2
Shingles	Skin lesions	Varicella zoster (Herpesvirus)
Cervical cancer	Viruses continuously released	Human papillomavirus
HIV/AIDS	Decreased CD4 T cells	HIV-1 and 2 (Lentivirus)
Liver cancer	Decreased cell growth	Hepatitis B virus
Progressive encephalitis	Mental deterioration	Rubella virus, Measles virus

Prions and Prion-Induced Diseases

Prions are proteinaceous infectious particles that cause spongiform encephalopathies.

• Diseases: Scrapie (sheep), BSE (cows), variant Creutzfeldt-Jakob disease and kuru (humans)

• Transmission: Eating infected material, transplants, contaminated surgical instruments

• Resistance: Prions cannot be destroyed by standard sterilization methods

Recap

• Differentiated a bacterium from a virus

• Described the general characteristics and morphology of viruses

• Characterized cytopathic effects of viral infection

• Summarized methods used for viral identification and cultivation

• Compared the multiplication patterns of bacterial and animal viruses

• Evaluated the effects of the lytic cycle and lysogeny on the host cell

• Discussed the link between viruses and cancer

• Described prions and prion-induced diseases