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Size Exclusion Chromatography definitions

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  • Size Exclusion Chromatography
    Technique using porous beads to separate proteins by size, with larger molecules eluting first due to their inability to enter bead cavities.
  • Gel Filtration Chromatography
    Alternative name for a method that employs gel-packed columns to filter proteins according to their molecular dimensions.
  • Column Chromatography
    General approach where a mixture passes through a packed column, enabling separation based on physical or chemical properties.
  • Stationary Phase
    Component inside the column, often made of gel beads, providing the environment for protein separation by size.
  • Porous Beads
    Gel particles with engineered cavities, forming the stationary phase and dictating protein movement based on molecular size.
  • Cavity
    Internal space within a bead, sized to allow entry of certain proteins, influencing their path and elution time.
  • Chromatogram
    Graphical output showing light absorbance versus elution time, visualizing the separation and order of protein elution.
  • Elution Time
    Duration required for a molecule to exit the column, shorter for larger proteins and longer for smaller ones.
  • Light Absorbance
    Measurement used to detect proteins as they elute, plotted on the chromatogram's y-axis.
  • Protein Mixture
    Sample containing proteins of various sizes, subjected to separation in the chromatography column.
  • Molecular Size
    Physical dimension of a protein, determining its ability to enter bead cavities and its elution order.
  • Unknown Protein
    Molecule whose size can be inferred by comparing its elution time to those of proteins with known dimensions.
  • Intermediate Protein
    Molecule with a size between large and small, partially entering bead cavities and eluting after larger proteins.
  • Small Protein
    Molecule able to enter most bead cavities, resulting in a longer path and slower elution from the column.
  • Large Protein
    Molecule too big for bead cavities, taking a direct route around beads and eluting first from the column.