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Electron Microscopy definitions

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  • Electron Microscopy
    Technique using electrons for visualization, enabling much higher resolution of cellular structures than light-based methods.
  • Resolution
    Smallest distance between two points that can be distinguished, reaching up to 0.002 nanometers with advanced imaging.
  • Specimen Preparation
    Process involving preservation and ultra-thin slicing to make biological samples suitable for high-resolution imaging.
  • Immuno Gold Staining
    Method labeling samples with a thin gold layer, making targeted regions appear black due to electron density.
  • Metal Shadowing
    Technique coating samples with metal from one angle, creating shadows that highlight surface contours and details.
  • Transmission Electron Microscopy
    Imaging approach where electrons pass through a sample, revealing internal structures with high clarity.
  • Scanning Electron Microscopy
    Method scanning a sample's surface with electrons, providing detailed images of external morphology.
  • Double Membrane
    Structural feature visible in organelles like mitochondria, consisting of two closely apposed lipid bilayers.
  • Electron Density
    Property causing certain regions to appear darker in images due to increased interaction with electrons.
  • Mitochondria
    Organelle whose intricate internal and external features are revealed in detail by advanced imaging techniques.
  • Magnification
    Degree to which an image is enlarged, reaching levels far beyond those possible with light-based instruments.
  • Ultrathin Section
    Extremely thin slice of a sample, typically 1/100th to 1/200th the thickness of a cell, essential for electron imaging.
  • Surface Detail
    Fine structural information about the exterior of a sample, best visualized using scanning techniques.
  • Antibody Labeling
    Strategy using antibodies conjugated with gold to specifically highlight proteins or organelles in imaging.
  • Cellular Structure
    Complex organization within cells, including membranes and organelles, made visible at high resolution.