Ch. 7 - DNA Structure and Replication

Sanders - Genetic Analysis: An Integrated Approach 3rd Edition

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Sanders 3rd Edition

Ch. 7 - DNA Structure and Replication

Problema 5b

Capitolo 7, Problema 5b

One strand of a fragment of duplex DNA has the sequence 5'-ATCGACCTGATC-3'.
What is the name of the bond that joins one nucleotide to another in the DNA strand?

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Understand that DNA is composed of nucleotides, which are joined together to form a strand. Each nucleotide consists of three components: a phosphate group, a sugar molecule (deoxyribose), and a nitrogenous base.

Recognize that the bond joining one nucleotide to another in a DNA strand is a covalent bond called a phosphodiester bond. This bond connects the phosphate group of one nucleotide to the 3' hydroxyl group of the sugar molecule in the next nucleotide.

Visualize the structure of the DNA strand: the backbone is made up of alternating sugar and phosphate groups, while the nitrogenous bases extend inward to form base pairs with the complementary strand.

Recall that the phosphodiester bond is formed during DNA synthesis by the enzyme DNA polymerase, which catalyzes the addition of nucleotides to the growing DNA strand.

Note that the directionality of the DNA strand is determined by the orientation of the phosphodiester bonds, which run from the 5' end (phosphate group) to the 3' end (hydroxyl group).

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Phosphodiester Bond

A phosphodiester bond is a type of covalent bond that links nucleotides together in a DNA strand. It forms between the phosphate group of one nucleotide and the hydroxyl group on the sugar of another nucleotide, creating a backbone that is essential for the structural integrity of DNA.

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DNA Structure

Nucleotide Structure

Nucleotides are the building blocks of DNA and consist of three components: a phosphate group, a five-carbon sugar (deoxyribose in DNA), and a nitrogenous base. The sequence of these nucleotides encodes genetic information, and understanding their structure is crucial for grasping how they connect through phosphodiester bonds.

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DNA Structure

DNA is structured as a double helix, composed of two strands running in opposite directions, held together by hydrogen bonds between complementary nitrogenous bases. The integrity of the DNA molecule relies on the phosphodiester bonds that form the sugar-phosphate backbone, allowing it to maintain its shape and function in genetic processes.

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DNA Structure

Pratica correlata

Domanda del libro di testo

Hershey and Chase selected the bacteriophage T2 for their experiment assessing the role of DNA in heredity because T2 contains protein and DNA, but not RNA. Explain why T2 was a good choice for this experiment.

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Domanda del libro di testo

One strand of a fragment of duplex DNA has the sequence 5'-ATCGACCTGATC-3'.

Which chemical groups of nucleotides react to form the bond in part (b)?

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Domanda del libro di testo

One strand of a fragment of duplex DNA has the sequence 5'-ATCGACCTGATC-3'.

What enzymes catalyze the reaction in part (d)?

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