You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissue, a genomic library made from DNA isolated from human muscle tissue, a human brain cDNA library, and a human muscle cDNA library.
Which of these would have the greatest diversity of sequences?

Ligase catalyzes a reaction between the 5′ phosphate and the 3′ hydroxyl groups at the ends of DNA molecules. The enzyme calf intestinal phosphatase catalyzes the removal of the 5′5′ phosphate from DNA molecules. What would be the consequence of treating a cloning vector, before ligation, with calf intestinal phosphatase?

Explain the meaning of 'identity by descent' in the context of identifying genealogical relationship between individuals. In these analyses, why are segments of chromosomes (haplotypes) rather than individual STRs used to identify genetic relationships?

You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissue, a genomic library made from DNA isolated from human muscle tissue, a human brain cDNA library, and a human muscle cDNA library.

Would the sequences contained in each library be expected to overlap completely, partially, or not at all with the sequences present in each of the other libraries?

What is the statistical principle underlying genetic health risk assessment? Why are these assessments not predictive of disease occurrence?

What is the exclusion principle? How is it used in forensic genetic analysis and in paternity determination?

Using the genomic libraries, you wish to clone the human gene encoding myostatin, which is expressed only in muscle cells.

Assuming the human genome is 3x10⁹ bp and that the average insert size in the genomic libraries is 100 kb, how frequently will a clone representing myostatin be found in the genomic library made from muscle?