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Microbiology Exam 1 Study Guide: Chapters 1, 3, 4, 6, 7

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Importance of Microorganisms

Scientific Naming of Organisms

Members of the Bacterial World

Introduction to Bacteria

Introduction to Archaea

Introduction to Eukarya

Introduction to Spontaneous Generation

Francesco Redi's Experiments

Needham vs. Spallanzani

Pasteur's Experiments on Spontaneous Generation

Koch's Postulates

Introduction to Microscopes

Magnification, Resolution, & Contrast

Introduction to Light Microscopy

Light Microscopy: Bright-Field Microscopes

Light Microscopes that Increase Contrast

Light Microscopes that Detect Fluorescence

Electron Microscopes

Introduction to Staining

Simple Staining

Differential Staining

Gram Stain

Cell Envelope & Biological Membranes

Bacterial & Eukaryotic Cell Membranes

Archaeal Cell Membranes

Types of Membrane Proteins

Concentration Gradients and Diffusion

Introduction to Membrane Transport

Passive vs. Active Transport

Osmosis

Simple and Facilitated Diffusion

Active Transport

Group Translocation

Prokaryotic & Eukaryotic Cells

Binary Fission

Generation Times

Bacterial Cell Morphology & Arrangements

Overview of Prokaryotic Cell Structure

Introduction to Bacterial Cell Walls

Gram-Positive Cell Walls

Gram-Negative Cell Walls

Gram-Positive vs. Gram-Negative Cell Walls

The Glycocalyx: Capsules & Slime Layers

Introduction to Biofilms

Pili

Fimbriae & Hami

Introduction to Prokaryotic Flagella

Prokaryotic Flagellar Structure

Prokaryotic Flagellar Movement

Prokaryotic Ribosomes

Cell Inclusions

Endospores

Sporulation

Germination

Introduction to Cultivating Microbial Growth

Microbial Growth Curves in a Closed System

Temperature Requirements for Microbial Growth

Oxygen Requirements for Microbial Growth

pH Requirements for Microbial Growth

Osmolarity Factors for Microbial Growth

Nutritional Factors of Microbial Growth

Growth Factors

Growing a Pure Culture

Types of Solid Culture Media

Plating Methods

Measuring Growth by Direct Cell Counts

Measuring Growth by Plate Counts

Measuring Growth by Membrane Filtration

Measuring Growth by Biomass

Introduction to the Types of Culture Media

Chemically Defined Media

Complex Media

Selective Media

Differential Media

Reducing Media

Enrichment Media

Introduction to Controlling Microbial Growth

Selecting a Method to Control Microbial Growth

Physical Methods to Control Microbial Growth

Chemical Methods to Control Microbial Growth

Chemicals Used to Control Microbial Growth

Liquid Chemicals: Alcohols, Aldehydes, & Biguanides

Liquid Chemicals: Halogens

Liquid Chemicals: Surface-Active Agents

Chemical Gases: Ethylene Oxide, Ozone, & Formaldehyde