What is the possible effect of a mutation causing the insertion of two nucleotides in the middle of the trpL region well before the polypeptide stop codon?

• A. The mutation could result in the production of a truncated TrpL protein, which could interfere with the regulation of the trp operon.
• B. The mutation could cause a frameshift, leading to the production of a completely different protein with a different function than the original TrpL protein.
• C. The mutation could cause premature termination of translation of the trpL gene, leading to the production of a non-functional or partially functional TrpL protein.
• D. All of the above.
• E. None of the above.

Answer: (D)