Q: Which equation best represents the relationship between sequence complexity and the number of repetitive elements in a genome?

$\text{Sequence Complexity} \propto \frac{1}{\text{Number of Repetitive Elements}}$

Question 1

Which of the following statements best describes the difference between prokaryotic and eukaryotic chromosomes?

Accepted Answer

Prokaryotic chromosomes are circular and typically have a single origin of replication, while eukaryotic chromosomes are linear and contain multiple origins of replication.

Question 2

During DNA replication in bacteria, which enzyme is responsible for unwinding the DNA double helix at the origin of replication?

Accepted Answer

Helicase

Question 3

Which model of DNA replication is supported by experimental evidence and describes each daughter DNA molecule as containing one parental and one newly synthesized strand?

Accepted Answer

Semiconservative replication

Question 4

A mutation disables the 3' to 5' exonuclease activity of DNA polymerase III in E. coli. What is the most likely consequence?

Accepted Answer

Proofreading of newly synthesized DNA will be impaired, increasing mutation rates.

Question 5

Which equation correctly represents the direction of DNA synthesis by DNA polymerases?

Accepted Answer

$5' \rightarrow 3'$

Question 6

In eukaryotic cells, which protein complex is primarily responsible for compacting DNA into nucleosomes?

Accepted Answer

Histone proteins

Question 7

Which of the following best explains why eukaryotic DNA replication is more complex than prokaryotic DNA replication?

Accepted Answer

Eukaryotic chromosomes are linear, contain more DNA, and are complexed with proteins.

Question 8

Which enzyme is responsible for relieving the increased supercoiling ahead of the replication fork during DNA replication?

Accepted Answer

DNA gyrase (topoisomerase II)

Question 9

A researcher isolates a DNA fragment that is highly repetitive and found in hundreds to thousands of copies in the genome. What type of DNA is this most likely to be?

Accepted Answer

Highly repetitive DNA

Question 10

Which of the following is NOT a function of DNA polymerase I in E. coli?

Accepted Answer

Sealing nicks between Okazaki fragments

Question 11

During homologous recombination, which structure is formed as a result of strand migration and pairing between homologous chromosomes?

Accepted Answer

Holliday junction

Question 12

Which histone type is most lysine-rich and has the highest molecular weight among the core histones?

Accepted Answer

H1

Question 13

If a bacterial cell loses the ability to produce single-stranded binding proteins (SSBPs), what is the most likely effect on DNA replication?

Accepted Answer

The template strands may reanneal, preventing proper replication.

Question 14

Which of the following best describes the role of chromatin remodeling in eukaryotic cells?

Accepted Answer

It exposes regions of DNA to allow replication and gene expression.

Question 15

Which process is catalyzed by RNA-dependent DNA polymerase (reverse transcriptase)?

Accepted Answer

Reverse transcription

Question 16

A DNA molecule undergoes positive supercoiling. What is the effect on the DNA helix?

Accepted Answer

The helix tightens and becomes more difficult to unwind.

Question 17

Which of the following is required for DNA polymerase to initiate DNA synthesis?

Accepted Answer

A free 3' hydroxyl group on a primer

Question 18

Which equation best represents the relationship between sequence complexity and the number of repetitive elements in a genome?

Accepted Answer

$	ext{Sequence Complexity} \propto \frac{1}{	ext{Number of Repetitive Elements}}$

Question 19

Which of the following is a property shared by both prokaryotic and eukaryotic DNA replication?

Accepted Answer

Both require a template, four deoxyribonucleoside triphosphates, and a primer.

Question 20

A patient is diagnosed with a genetic disorder caused by a mutation in the gene encoding DNA ligase. Which step of DNA replication is most likely affected?

Accepted Answer

Sealing nicks between adjacent DNA fragments

DNA Replication, Recombination, and Chromatin: Structure and Function in Prokaryotes and Eukaryotes

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