Chromatin acetylation is experimentally increased at multiple loci across a cell line. Analyze and predict the most likely pattern of gene expression and a plausible dietary metabolite that might contribute to increased acetylation.

• A. Acetylation exclusively targets tRNA molecules to alter their charging, and dietary cholesterol is the primary acetyl donor that causes targeted tRNA acetylation and corresponding changes in ribosomal fidelity.
• B. Chromatin acetylation degrades histones and thereby reduces transcription irreversibly, with dietary trans fats serving as dominant acetyl donors that cause permanent gene silencing.
• C. Increased acetylation compacts chromatin and universally silences genes; glucose-6-phosphate directly acetylates DNA bases to reduce transcription.
• D. Increased acetylation usually opens chromatin and enhances gene transcription at those loci, raising protein production; acetyl-CoA derived from carbohydrate and fatty acid metabolism is a key metabolite that donates acetyl groups and can influence histone acetylation.

Answer: (D)