Analytical Chemistry
What is the isoelectric point of a molecule?
Calculate the isoelectric point of a diprotic acid with pKa1 = 3.5 and pKa2 = 5.5.
Given the pKa values of histidine as 1.8, 6.0, and 9.2, calculate its isoelectric point.
What is the significance of the isoelectric point in protein purification?
How does the charge of an amino acid change when the pH is below its isoelectric point?