Freeman 7th Edition
Ch. 20 - The Molecular Revolution: Biotechnology, Genomics, and New Frontiers
Problem 4The human genome size is 3 billion base pairs, and the size of the baker's yeast genome, a single-celled organism, is 12 million base pairs. Therefore, the predicted genome size for another single-celled organism, an amoeba,
a. Is about the size of the human genome
b. Is about the size of the yeast genome
c. Is somewhere between the sizes of the yeast and human genomes
d. Cannot be predicted with any certainty
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Key Concepts
Genome Size Variation
C-value Paradox
Amoeba Genome Size
What is a plasmid?
a. An organelle found in many bacteria and certain eukaryotes
b. A circular DNA molecule that replicates independently of the main chromosome(s)
c. A type of virus that has a DNA genome and infects certain types of human cells, including lung and respiratory tract tissue
d. A type of virus that has an RNA genome, codes for reverse transcriptase, and inserts a cDNA copy of its genome into cells
After finding a gene that causes a disease, researchers often introduce the defective allele into mice to create an animal model of the disease. Why are these models valuable?
a. They allow the testing of potential drug therapies without endangering human patients.
b. They allow the sequencing of the mutant allele.
c. They allow the production of large quantities of the defective gene product, usually a protein.
d. They allow the study of how the gene was transmitted from parents to offspring.
Explain how RNA-seq can be used to analyze patterns of gene expression.
Consider the validity of the following statements about genome editing. Select True or False for each statement.
T/FCas proteins work as endonucleases.
T/FsgRNA is used by bacterial cells to detect which DNA to cut.
T/FHomologous recombination is always used to join pieces of broken DNA.
T/FIt is possible to modify genes as well as disrupt them by genome editing.
Gene density is the number of genes per million base pairs (Mbp). Using Figure 20.5b, find the approximate number of genes estimated in water fleas and in humans, and note the size of each genome. Calculate the gene density in water fleas relative to that in humans.