What do restriction endonucleases do?

Question

Accepted Answer

Hi everyone here's our next question identify the incorrect statement about restriction and the nucleus is. So let's recall from our content videos that restriction under nucleus is are enzymes that cut D. N. A. At specific base pair sequences. They're called restriction under nucleus is because they protect bacteria by cutting up viral D. N. A. To protect the bacteria from infection when they're infected by bacteriophages. But they're useful tools and recombinant DNA technology. Since we know the sequence that gets cut allows you to cut open circular bacterial D. N. A. And insert sequences by designing them with ends that correspond to the cut ends. So just as a little reminder diagram here An example of one of them, one that's used quite commonly in molecular biology. The restriction enzyme echo r. one. It cleaves the D. N. A. And this particular sequence here as located which leaves the D. N. A. With two what we call sticky ends that look like this. So if you design something to insert with complimentary ends you can insert a piece of bacteria in here. So let's look at our answer choices with that in mind. Choice A. Says these are natural enzymes of bacteria. Yes that is correct. That's true. So not our answer. Since we're looking for an incorrect statement, they do occur naturally in bacterias to help protect them from viruses choice B says they act as molecular scissors. That is true. Also there used by molecular biologist to cut things deliberately and back in their natural environment. They cut up viral D. N. A. To protect the bacteria. So that is correct. And therefore not our answer. Choice C. Says there ended nucleus. Is that cut double stranded DNA? Yes, that is true. They cut double stranded DNA in specific sequences so that is correct and not our answer. So finally, we're on a choice D. These are enzymes that problem arise the D. N. A. Well, nope, that is not what they do. That is incorrect. So that is our answer again. They cut double stranded DNA. Not problem arise it. So our answer is the incorrect statement about restriction under new places. This choice D. These are enzymes that commemorates the D. N. A. Problem arise the D. N. A. Excuse me. See you in the next video.

What do restriction endonucleases do?

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Key Concepts

Restriction Endonucleases

Recognition Sites

Applications in Biotechnology