Microbiology: A Laboratory Manual, 12th edition

Published by Pearson (February 1, 2019) © 2020

  • James G. Cappuccino SUNY, Rockland Community College
  • Chad T. Welsh Lindenwood University

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For courses in microbiology lab and nursing and allied health microbiology lab.

A clinical and critical-thinking emphasis

Microbiology: A Laboratory Manual provides a solid underpinning of microbiology laboratory work with a strong focus on clinical applications and critical-thinking skills. The lab manual is clear, comprehensive, and versatile, and easily adapted to any microbiology lab course and paired with any undergraduate microbiology text.

The 12th Edition is extensively updated to enhance the student experience and meet instructor requirements. Updates and additions include clinical case studies, equipment and material checklists, new experiments, governing body guidelines and more.

Hallmark features of this title

  • Modular experiments allow instructors to easily select the type, complexity, and order of experiments they wish to cover.
  • Clinical Applications integrated into each lab help students understand why they're learning each technique and connect them to possible future careers as nurses or in allied health fields.
  • The clinically relevant experiment, Propagation of Isolated Bacteriophage Cultures, guides students in isolating and preparing bacteriophages for genetic manipulation.
  • Further Reading sections appear in each experiment or part and point students to related content in their microbiology text.
  • Instructor resources include laboratory safety protocols, organizational lists, and review question answers.

New and updated features of this title

  • RETURNED: Handwashing is the first experiment and has been re-added to the manual in response to popular demand.
  • Published laboratory standards from numerous governing bodies, including the USDA and the FSIS, are added to experiment introductions to provide students with various industry standards and regulations.
  • Clinical case studies review a fictitious case in each section and illustrate the importance of the lab science in those experiments.
  • Detection of Enteric Bacteria on Raw Meat experiment is based on USDA and FSIS protocols for cultivating and identifying bacteria on commercially prepared meat.
  • NEW DESIGN: Isolation of Fungal species experiment illustrates the methodology for isolating fungal species from an environmental sample.
  • REVISED: Material, media, and organism lists have been converted to easy-to-follow checklists, allowing students to better prepare and focus on experiments.

Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural

  • Experiment 1: Effectiveness of Hand Washing
  • Experiment 2: Culture Transfer Techniques
  • Experiment 3: Techniques for Isolation of Pure Cultures
  • Experiment 4: Cultural Characteristics of Microorganisms

Part 2 Microscopy

  • Experiment 5: Microscopic Examination of Stained Cell Preparations
  • Experiment 6:Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

Part 3 Bacterial Staining

  • Experiment 7: Preparation of Bacterial Smears
  • Experiment 8: Simple Staining
  • Experiment 9: Negative Staining
  • Experiment 10:Gram Stain
  • Experiment 11: Acid-Fast Stain
  • Experiment 12: Differential Staining for Visualization of Bacterial Cell Structures

Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations

  • Experiment 13:Nutritional Requirements: Media for the Routine Cultivation of Bacteria
  • Experiment 14: Use of Differential, Selective, and Enriched Media
  • Experiment 15: Physical Factors: Temperature
  • Experiment 16: Physical Factors: pH of the Extracellular Environment
  • Experiment 17: Physical Factors: Atmospheric Oxygen Requirements
  • Experiment 18: Techniques for the Cultivation of Anaerobic Microorganisms
  • Experiment 19: Serial Dilution–Agar Plate Procedure to Quantitate Viable Cells
  • Experiment 20: The Bacterial Growth Curve

Part 5 Biochemical Activities of Microorganisms

  • Experiment 21: Extracellular Enzymatic Activities of Microorganisms
  • Experiment 22: Carbohydrate Fermentation
  • Experiment 23: Triple Sugar–Iron Agar Test
  • Experiment 24: IMViC Test
  • Experiment 25: Hydrogen Sulfide Test
  • Experiment 26: Urease Test
  • Experiment 27: Litmus–Milk Reactions
  • Experiment 28: Nitrate Reduction Test
  • Experiment 29: Catalase Test
  • Experiment 30: Oxidase Test
  • Experiment 31: Utilization of Amino Acids
  • Experiment 32: Genus Identification of Unknown Bacterial Cultures

Part 6 The Protozoa

  • Experiment 33: Free-Living Protozoa
  • Experiment 34: Parasitic Protozoa

Part 7 The Fungi

  • Experiment 35: Cultivation and Morphology of Molds
  • Experiment 36: Isolation of a Soil Fungal Species
  • Experiment 37:Morphology, Cultural Characteristics and Reproduction

Part 8 The Viruses

  • Experiment 38: Cultivation and Enumeration of Bacteriophages
  • Experiment 39: Isolation of Coliphages from Raw Sewage
  • Experiment 40: Propagation of Isolated Bacteriophage Cultures

Part 9 Physical and Chemical Agents for the Control of Microbial Growth

  • Experiment 41: Physical Agents of Control: Moist Heat
  • Experiment 42: Chemical Agents of Control: Chemotherapeutic Agents
  • Experiment 43: Determination of Penicillin Activity in the Presence and Absence of Penicillinase
  • Experiment 44: Chemical Agents of Control: Disinfectants and Antiseptics

Part 10 Microbiology of Food

  • Experiment 45: Microbiological Analysis of Food Products: Bacterial Count
  • Experiment 46: Isolation of Salmonella from Raw Meat
  • Experiment 47: Microbial Fermentation

PART 11 Microbiology of Water

  • Experiment 48: Standard Qualitative Analysis of Water
  • Experiment 49: Quantitative Analysis of Water: Membrane Filter Method

PART 12 Microbiology of Soil

  • Experiment 50: Microbial Populations in Soil: Enumeration
  • Experiment 51: Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
  • Experiment 52: Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

PART 13 Bacterial Genetics

  • Experiment 53: Enzyme Induction
  • Experiment 54: Bacterial Conjugation
  • Experiment 55: Isolation of a Streptomycin-Resistant Mutant
  • Experiment 56: The Ames Test: A Bacterial Test System for Chemical Carcinogenicity
  • Experiment 57: Utilization of Bacterial Plasmids
  • Experiment 58: Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA

PART 14 Medical Microbiology

  • Experiment 59: Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries
  • Experiment 60: Normal Microbial Flora of the Throat and Skin
  • Experiment 61: Identification of Human Staphylococcal Pathogens
  • Experiment 62: Identification of Human Streptococcal Pathogens
  • Experiment 63: Identification of Streptococcus pneumoniae
  • Experiment 64: Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems
  • Experiment 65: Isolation and Presumptive Identification of Campylobacter
  • Experiment 66: Microbiological Analysis of Urine Specimens
  • Experiment 67: Microbiological Analysis of Blood Specimens
  • Experiment 68: Species Identification of Unknown Bacterial Cultures

PART 15 Immunology

  • Experiment 69: Precipitin Reaction: The Ring Test
  • Experiment 70: Agglutination Reaction: The Febrile Antibody Test
  • Experiment 71: Enzyme-Linked Immunosorbent Assay
  • Experiment 72: Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures

About our authors

In Memoriam: James G. Cappuccino (1930–2018)

James G. Cappuccino is a retired professor emeritus of microbiology from the Department of Biology of the State University of New York at Rockland Community College in Suffern, New York. He received his B.S degree from Seton Hall University in 1951, his M.S degree (1955) and his Ph.D. (1957) in microbiology from Rutgers University in New Brunswick, New Jersey. He was the author and co-author of numerous papers in the area of cancer research, and was a member of the faculty of the Sloan Kettering Division of the Graduate school of Medical Sciences at Cornell University where he taught microbiology from 1957-1970. From there, he taught microbiology, parasitology and clinical chemistry at SUNY Rockland until 2008. He was awarded the status of emeritus professor in 2012. In 1991 he was the recipient of the Chancellor's award from the State University of New York for Excellence in Teaching. He is an emeritus member of the American Society for Cancer Research (ASCR) and an emeritus member of American society for Microbiology (ASM). When not writing he enjoys spending time with his wife Elaine and their family at their summer home at the New Jersey shore. He also enjoys theater, literature, and the quiet hour in his wood working shop.

Chad T. Welsh holds a Ph.D. in Microbiology and Immunology from the University of Louisville, School of Medicine, also an M.S. and B.S. in Biology from Middle Tennessee State University. Currently he is the Chair of the Division of Biological and Earth Sciences at Lindenwood University in St. Charles, MO where he has the privilege of teaching Microbiology, both for non-majors and majors, Cellular Immunology, Parasitology, and many other courses since 2010. His research interests fall within bacteriology, eukaryotic cell biology, and immunology, focusing primarily on intracellular eukaryotic signals in response to pulmonary bacterial pathogens. His mentored research projects with his students have spanned the interest areas of soil microbial ecology, immune stress responses in collegiate athletes, oral bacterial flora communities, and many others.

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