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Ch. 18 - Developmental Genetics
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 18, Problem 19

You are traveling in the Netherlands and overhear a tulip breeder describe a puzzling event. Tulips normally have two outer whorls of brightly colored petal-like organs, a third whorl of stamens, and an inner (fourth) whorl of carpels. However, the breeder found a recessive mutant in his field in which the outer two whorls were green and sepal-like, whereas the third and fourth whorls both contained carpels. What can you speculate about the nature of the gene that was mutated?

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Step 1: Begin by understanding the normal floral organ arrangement in tulips. Tulips typically have four whorls: (1) outer whorl of sepals, (2) second whorl of petals, (3) third whorl of stamens, and (4) inner whorl of carpels. Each whorl is determined by specific gene expression patterns.
Step 2: Analyze the mutant phenotype described. In the mutant tulip, the outer two whorls are green and sepal-like, while the third and fourth whorls both contain carpels. This suggests a disruption in the normal differentiation of floral organs.
Step 3: Consider the ABC model of flower development, which explains how three classes of genes (A, B, and C) control the identity of floral organs. Class A genes specify sepals, Class A and B together specify petals, Class B and C together specify stamens, and Class C alone specifies carpels.
Step 4: Based on the mutant phenotype, hypothesize that the mutation likely affects a Class B gene. If Class B gene function is lost, petals (second whorl) and stamens (third whorl) fail to develop, and instead, sepals (Class A activity) and carpels (Class C activity) are formed in their place.
Step 5: Conclude that the gene mutated in this tulip is likely a Class B gene, which is essential for the development of petals and stamens. The recessive nature of the mutation indicates that both copies of the gene must be non-functional for the mutant phenotype to appear.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Flower Structure and Whorls

Flowers are typically organized into whorls, which are layers of floral organs. The outer whorls usually consist of sepals and petals, while the inner whorls contain stamens and carpels. Understanding this structure is crucial for analyzing mutations, as changes in specific whorls can indicate alterations in developmental pathways.
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Dominance and Recessiveness in Genetics

In genetics, traits are often determined by alleles, which can be dominant or recessive. A recessive allele only expresses its trait when two copies are present. The observation of green, sepal-like outer whorls in the tulip suggests that the mutation is recessive, as it alters the typical phenotype when homozygous.
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Homeotic Genes and Floral Development

Homeotic genes are critical in determining the identity of floral organs during development. Mutations in these genes can lead to the transformation of one type of organ into another, such as carpels developing in place of stamens or petals. The tulip breeder's observation of carpels in the third and fourth whorls suggests a potential mutation in homeotic genes that regulate floral organ identity.
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Related Practice
Textbook Question

The pair-rule gene fushi tarazu is expressed in the seven even-numbered parasegments during Drosophila embryogenesis. In contrast, the segment polarity gene engrailed is expressed in the anterior part of each of the 14 parasegments. Since both genes are active at similar times and places during development, it is possible that the expression of one gene is required for the expression of the other. This can be tested by examining the expression of the genes in a mutant background—for example, looking at fushi tarazu expression in an engrailed mutant background, and vice versa. Based on your prediction, can you predict the phenotype of the fushi tarazu and engrailed double mutant?

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Textbook Question

In contrast to Drosophila, some insects (e.g., centipedes) have legs on almost every segment posterior to the head. Based on your knowledge of Drosophila, propose a genetic explanation for this phenotype, and describe the expected expression patterns of genes of the Antennapedia and bithorax complexes.

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Textbook Question
The bristles that develop from the epidermis in Drosophila are evenly spaced, so that two bristles never occur immediately adjacent to each other. How might this pattern be established during development?
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Textbook Question

A powerful approach to identifying genes of a developmental pathway is to screen for mutations that suppress or enhance the phenotype of interest. This approach was undertaken to elucidate the genetic pathway controlling C. elegans vulval development. A lin-3 loss-of-function mutant with a vulva-less phenotype was mutagenized. Based on your knowledge of the genetic pathway, what types of mutations will suppress the vulva-less phenotype?

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Textbook Question

A powerful approach to identifying genes of a developmental pathway is to screen for mutations that suppress or enhance the phenotype of interest. This approach was undertaken to elucidate the genetic pathway controlling C. elegans vulval development. In a complementary experiment, a gain-of-function let-23 mutant with a multi-vulva phenotype was also mutagenized. What types of mutations will suppress the multi-vulva phenotype?

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Textbook Question

The Hoxd9–13 genes are thought to specify digit identity. What would be the consequence of ectopically expressing Hoxd10 throughout the developing mouse limb bud? What about Hoxd11? What about both Hoxd10 and Hoxd11?

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