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Laboratory Experiments in Microbiology, 12th edition

Published by Pearson (July 14, 2021) © 2019

  • Ted R. Johnson St. Olaf College
  • Christine L. Case Skyline College
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Laboratory Experiments in Microbiology features 57 class-tested, customizable exercises that teach basic microbiology techniques and applications. The manual provides coverage of every area of microbiology for undergraduate students across diverse disciplines. The perfect companion to Tortora/Funke/Case's Microbiology: An Introduction, it can be used with any introductory microbiology text.

The 12th Edition indicates when an exercise addresses the human or environmental microbiome or an ASM guideline or is investigative. New ASM Thinking Skills outline steps to help students develop laboratory thinking skills.   

PART ONE MICROSCOPY

  1. Use and Care of the Microscope
  2. Examination of Living Microorganisms

PART TWO HANDLING BACTERIA

  1. Microbes in the Environment
  2. Transfer of Bacteria: Aseptic Technique

PART THREE STAINING METHODS

  1. Preparation of Smears and Simple Staining
  2. Negative Staining
  3. Gram Staining
  4. Acid-Fast Staining
  5. Structural Stains (Endospore, Capsule, and Flagella)
  6. Morphological Unknown

PART FOUR CULTIVATION OF BACTERIA

  1. Isolation of Bacteria by Dilution Techniques
  2. Special Media for Isolating Bacteria

PART FIVE MICROBIAL METABOLISM

  1. Carbohydrate Catabolism
  2. Fermentation
  3. Protein Catabolism, Part 1
  4. Protein Catabolism, Part 2
  5. Respiration
  6. Unknown Identification and Bergey's Manual

PART SIX MICROBIAL GROWTH

  1. Oxygen and the Growth of Bacteria
  2. Determination of a Bacterial Growth Curve: The Role of Temperature
  3. Biofilms

PART SEVEN CONTROL OF MICROBIAL GROWTH

  1. Physical Methods of Control: Heat
  2. Physical Methods of Control: Ultraviolet Radiation
  3. Chemical Methods of Control: Disinfectants and Antiseptics
  4. Chemical Methods of Control: Antimicrobial Drugs
  5. Effectiveness of Hand Scrubbing

PART EIGHT MICROBIAL GENETICS

  1. Regulation of Gene Expression
  2. Isolation of Bacterial Mutants
  3. Transformation of Bacteria
  4. DNA Fingerprinting
  5. Genetic Engineering
  6. Ames Test for Detecting Possible Chemical Carcinogens

PART NINE THE MICROBIAL WORLD

  1. Fungi: Yeasts and Molds
  2. Phototrophs: Algae and Cyanobacteria
  3. Protozoa
  4. Parasitic Helminths

PART TEN VIRUSES

  1. Isolation and Titration of Bacteriophages
  2. Plant Viruses

PART ELEVEN INTERACTION OF MICROBE AND HOST

  1. Epidemiology
  2. Koch's Postulate

PART TWELVE IMMUNOLOGY

  1. Innate Immunity
  2. Agglutination Reactions: Slide Agglutination
  3. Agglutination Reactions: Microtiter Agglutination
  4. ELISA Technique

PART THIRTEEN MICRORGANISMS AND DISEASE

  1. Bacteria of the Skin
  2. Bacteria of the Respiratory Tract
  3. Bacteria of the Mouth
  4. Bacteria of the Gastrointestinal Tract
  5. Bacteria of the Urogenital Trac
  6. Identification of an Unknown from a Clinical Sample
  7. Rapid Identification Methods

PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT

  1. Microbes in Water: Multiple-Tube Technique
  2. Microbes in Water: Membrane Filter Technique
  3. Microbes in Food: Contamination
  4. Microbes Used in the Production of Foods
  5. Microbes in Soil: The Nitrogen and Sulfur Cycles
  6. Microbes in Soil: Bioremediation

APPENDICES

  • A. Pipetting
  • B. Dilution Techniques and Calculations
  • C. Use of the Spectrophotometer
  • D. Graphing
  • E. Use of the Dissecting Microscope
  • F. Use of the Membrane Filter
  • G. Electrophoresis
  • H. Keys to Bacteria

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