Laboratory Experiments in Microbiology, 12th edition

Published by Pearson (January 1, 2018) © 2019

  • Ted R. Johnson St. Olaf College
  • Christine L. Case Skyline College

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For general microbiology laboratory courses.

Class-tested, customizable exercises

Laboratory Experiments in Microbiology features 57 class-tested, customizable exercises that teach basic microbiology techniques and applications. The manual provides coverage of every area of microbiology for undergraduate students across diverse disciplines. The perfect companion to Tortora/Funke/Case's Microbiology: An Introduction, it can be used with any introductory microbiology text.

The 12th Edition indicates when an exercise addresses the human or environmental microbiome or an ASM guideline or is investigative. New ASM Thinking Skills outline steps to help students develop laboratory thinking skills.

Hallmark features of this title

  • Case Studies open each part, introducing a real-world scenario or health-oriented case in a real-world context. If applicable, the cases show how the exercises are medically relevant.
  • ASM BSL-2 safety lab protocol show which procedural steps and alternate bacteria are more appropriate for BSL-2 facilities.
  • Laboratory Reports close each exercise and help students learn to collect, record and present data systematically.
  • Conclusions section features investigative questions, also in the Lab Report, and encourage students to closely analyze and understand their lab exercise results.
  • Questions in Background, Procedures, and Lab Report sections emphasize critical-thinking skills, requiring students to recall and apply information using analysis and logical reasoning.

New and additional features of this title

  • ASM Thinking Skills promote the following Laboratory Thinking skills: 1) predict expected results, 2) follow an experimental protocol, 3) collect and organize data, 4) draw appropriate conclusions based on the results, 5) work effectively in teams or groups so that the task, results, and analysis are shared, 6) effectively manage time and tasks allowing concurrent and/or overlapping tasks to be done simultaneously by individuals and in a group
  • Icons identify exercises that address specific topics or techniques, such as an ASM guideline , the human or environmental microbiome or are investigative.
  • 100 photographs in full color, numerous diagrams and drawings help students in visualizing techniques and interpreting results.
  • Instructor's Preparation Guide by the authors includes specific instructions for preparing each lab, safety guidelines for handling blood and bodily fluids, and page references to Tortora, Funke, and Case's Microbiology: An Introduction.

PART ONE MICROSCOPY

  1. Use and Care of the Microscope
  2. Examination of Living Microorganisms

PART TWO HANDLING BACTERIA

  1. Microbes in the Environment
  2. Transfer of Bacteria: Aseptic Technique

PART THREE STAINING METHODS

  1. Preparation of Smears and Simple Staining
  2. Negative Staining
  3. Gram Staining
  4. Acid-Fast Staining
  5. Structural Stains (Endospore, Capsule, and Flagella)
  6. Morphological Unknown

PART FOUR CULTIVATION OF BACTERIA

  1. Isolation of Bacteria by Dilution Techniques
  2. Special Media for Isolating Bacteria

PART FIVE MICROBIAL METABOLISM

  1. Carbohydrate Catabolism
  2. Fermentation
  3. Protein Catabolism, Part 1
  4. Protein Catabolism, Part 2
  5. Respiration
  6. Unknown Identification and Bergey's Manual

PART SIX MICROBIAL GROWTH

  1. Oxygen and the Growth of Bacteria
  2. Determination of a Bacterial Growth Curve: The Role of Temperature
  3. Biofilms

PART SEVEN CONTROL OF MICROBIAL GROWTH

  1. Physical Methods of Control: Heat
  2. Physical Methods of Control: Ultraviolet Radiation
  3. Chemical Methods of Control: Disinfectants and Antiseptics
  4. Chemical Methods of Control: Antimicrobial Drugs
  5. Effectiveness of Hand Scrubbing

PART EIGHT MICROBIAL GENETICS

  1. Regulation of Gene Expression
  2. Isolation of Bacterial Mutants
  3. Transformation of Bacteria
  4. DNA Fingerprinting
  5. Genetic Engineering
  6. Ames Test for Detecting Possible Chemical Carcinogens

PART NINE THE MICROBIAL WORLD

  1. Fungi: Yeasts and Molds
  2. Phototrophs: Algae and Cyanobacteria
  3. Protozoa
  4. Parasitic Helminths

PART TEN VIRUSES

  1. Isolation and Titration of Bacteriophages
  2. Plant Viruses

PART ELEVEN INTERACTION OF MICROBE AND HOST

  1. Epidemiology
  2. Koch's Postulate

PART TWELVE IMMUNOLOGY

  1. Innate Immunity
  2. Agglutination Reactions: Slide Agglutination
  3. Agglutination Reactions: Microtiter Agglutination
  4. ELISA Technique

PART THIRTEEN MICRORGANISMS AND DISEASE

  1. Bacteria of the Skin
  2. Bacteria of the Respiratory Tract
  3. Bacteria of the Mouth
  4. Bacteria of the Gastrointestinal Tract
  5. Bacteria of the Urogenital Trac
  6. Identification of an Unknown from a Clinical Sample
  7. Rapid Identification Methods

PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT

  1. Microbes in Water: Multiple-Tube Technique
  2. Microbes in Water: Membrane Filter Technique
  3. Microbes in Food: Contamination
  4. Microbes Used in the Production of Foods
  5. Microbes in Soil: The Nitrogen and Sulfur Cycles
  6. Microbes in Soil: Bioremediation

APPENDICES

  • A. Pipetting
  • B. Dilution Techniques and Calculations
  • C. Use of the Spectrophotometer
  • D. Graphing
  • E. Use of the Dissecting Microscope
  • F. Use of the Membrane Filter
  • G. Electrophoresis
  • H. Keys to Bacteria

About our authors

Ted R. Johnson is a professor emeritus of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he has taught courses in microbiology and immunology. He has taught at St. Olaf for 39 years and previously taught at Mankato State University for five years. He received his master's degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several semester abroad and interim programs. He has been involved in advising health professions students and received the Fr.Joseph Walter Award for Health Profession advising. His research focuses on the immune response to virally induced cancer in various animal models.

Christine L. Case is a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 47 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.

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