Textbook Question
Restriction sites are palindromic; that is, they read the same in the 5' to 3' direction on each strand of DNA. What is the advantage of having restriction sites organized this way?
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Ch. 17 - Recombinant DNA Technology
Klug10th EditionEssentials of GeneticsISBN: 9780135588789
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Table of contents
- Ch. 1 - Introduction to Genetics16
- Ch. 2 - Mitosis and Meiosis28
- Ch. 3 - Mendelian Genetics37
- Ch. 4 - Modification of Mendelian Ratios53
- Ch. 5 - Sex Determination and Sex Chromosomes32
- Ch. 6 - Chromosome Mutations: Variation in Number and Arrangement37
- Ch. 7 - Linkage and Chromosome Mapping in Eukaryotes36
- Ch. 8 - Genetic Analysis and Mapping in Bacteria and Bacteriophages24
- Ch. 9 - DNA Structure and Analysis38
- Ch. 10 - DNA Replication29
- Ch. 11 - Chromosome Structure and DNA Sequence Organization22
- Ch. 12 - The Genetic Code and Transcription36
- Ch. 13 - Translation and Proteins27
- Ch. 14 - Gene Mutation, DNA Repair, and Transposition34
- Ch. 15 - Regulation of Gene Expression in Bacteria22
- Ch. 16 - Regulation of Gene Expression in Eukaryotes37
- Ch. 17 - Recombinant DNA Technology27
- Ch. 18 - Genomics, Bioinformatics, and Proteomics30
- Ch. 19 - The Genetics of Cancer21
- Ch. 20 - Quantitative Genetics and Multifactorial Traits37
- Ch. 21 - Population and Evolutionary Genetics45
Chapter 17, Problem 11
In the context of recombinant DNA technology, of what use is a probe?
Verified step by step guidance1
Understand that in recombinant DNA technology, a probe is a single-stranded DNA or RNA sequence that is complementary to a target sequence of interest.
Recognize that the probe is labeled with a detectable marker, such as a radioactive isotope or a fluorescent dye, to allow visualization after hybridization.
Use the probe to hybridize (bind) specifically to the complementary DNA or RNA sequence within a mixture of nucleic acids, enabling identification of the target sequence.
Apply the probe in techniques such as Southern blotting or Northern blotting to locate specific DNA or RNA fragments on a membrane after gel electrophoresis.
Interpret the presence and position of the probe's signal to determine whether the target sequence is present and to analyze its size or abundance.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Recombinant DNA Technology
Recombinant DNA technology involves combining DNA from different sources to create new genetic combinations. It is widely used in genetic engineering, cloning, and gene analysis to study or manipulate genes for research, medicine, and agriculture.
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Recombination after Single Strand Breaks
DNA Probe
A DNA probe is a short, single-stranded sequence of nucleotides labeled with a detectable marker. It is designed to hybridize specifically to a complementary DNA sequence, allowing researchers to locate or identify particular genes or DNA fragments within a complex mixture.
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Hybridization in Molecular Biology
Hybridization refers to the process where complementary nucleic acid strands pair by base-pairing. In recombinant DNA technology, probes hybridize to target sequences, enabling detection or isolation of specific DNA segments through techniques like Southern blotting or in situ hybridization.
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Related Practice
Textbook Question
List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?
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Textbook Question
What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?
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Textbook Question
If you performed a PCR experiment starting with only one copy of double-stranded DNA, approximately how many DNA molecules would be present in the reaction tube after 15 cycles of amplification?
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Textbook Question
In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges
(a) 92-26 °C
(b) 45-65 °C and
(c) 65-75 °C
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Textbook Question
We usually think of enzymes as being most active at around 37°C, yet in PCR the DNA polymerase is subjected to multiple exposures of relatively high temperatures and seems to function appropriately at 65–75°C. What is special about the DNA polymerase typically used in PCR?
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