Assume that you are delivering a solution sample from a pipette. Figures (a) and (b) show the volume level before and after dispensing the sample, respectively. State the liquid level (in mL) before and after dispensing the sample, and calculate the volume of the sample.
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Verified step by step guidance

1. Step 1: Observe the liquid level in figure (a), labeled 'START'. The liquid level is at 0.3 mL.
2. Step 2: Observe the liquid level in figure (b), labeled 'STOP'. The liquid level is at 0.2 mL.
3. Step 3: To calculate the volume of the sample dispensed, subtract the liquid level after dispensing (STOP) from the liquid level before dispensing (START). Use the formula: Volume dispensed = START volume - STOP volume.
4. Step 4: Substitute the observed values into the formula: Volume dispensed = 0.3 mL - 0.2 mL.
5. Step 5: Perform the subtraction to determine the volume of the sample dispensed. The result will be the volume in mL.

Key Concepts

Graduated Cylinder Measurement

Graduated cylinders are used to measure the volume of liquids accurately. The scale on the cylinder indicates the volume at various levels, typically in milliliters (mL). Understanding how to read these measurements is crucial for determining the liquid levels before and after dispensing a sample.

Volume Calculation

Volume calculation involves determining the difference in liquid levels before and after dispensing a sample. This is done by subtracting the final volume from the initial volume, providing the volume of the liquid dispensed. Accurate calculations are essential for precise scientific measurements.

Pipetting Technique

Pipetting is a technique used to transfer a specific volume of liquid from one container to another. Proper pipetting technique ensures that the correct volume is dispensed, which is critical in experiments and laboratory settings. Understanding how to use a pipette effectively is fundamental for accurate liquid handling.