Problem SA5
Why is Latin used in taxonomic nomenclature?
Problem S6
Give three characteristics of a “specific epithet.”
Problem SA7
How does the study of the nucleotide sequences of ribosomal RNA fit into a discussion of taxonomy?
Problem TF5
For each of the following statements that is true, write “true” in the blank. For each statement that is false, write the word(s) that should be substituted for the underlined word(s) to make the statement correct.
__________ Archaea are classified into phyla based primarily on tRNA sequences.
Problem VI2
Label the microscope.
Problem SA1
Explain how the principle, “electrons travel as waves,” applies to microscopy.
Problem SA2
Critique the following definition of magnification given by a student on a microbiology test: “Magnification makes things bigger.”
Problem SA3
Why can electron microscopes magnify only dead organisms?
Problem SA4
Put the following substances in the order they are used in a Gram stain:
Counterstain
Decolorizing agent
Mordant
Primary stain
Problem SA6
Explain why Gram-positive mycoplasmas appear pink in a Gram-stained smear.
Problem SA8
An atomic force microscope can magnify a living cell, whereas electron microscopes and scanning tunneling microscopes cannot. What requirement of scanning tunneling microscopes precludes the imaging of living specimens?
Problem 1a
Which of the following is smallest?
a. decimeter
d. millimeter
c. nanometer
d. micrometer
Problem 2a
A nanometer is _______ than a micrometer.
a. 10 times larger
b. 10 times smaller
c. 1000 times larger
d. 1000 times smaller
Problem 3a
Resolution is best described as the ____________.
a. ability to view something that is small
b. ability to magnify a specimen
c. ability to distinguish between two adjacent objects
d. difference between two waves of electromagnetic radiation
Problem 4a
Curved glass lenses _______light.
a. refract
b. bend
c. magnify
d. both a and b
Problem 4.1a
If an objective magnifies 40× and each binocular lens magnifies 15×, the total magnification of the object being viewed is ________.
Problem 4.1a
Label each photograph with the type of microscope used to acquire the image.
a. _______<IMAGE>
b. _______<IMAGE>
c. _______<IMAGE>
d. _______<IMAGE>
e. _______<IMAGE>
f. _______<IMAGE>
Problem 4.2a
The type of fixation developed by Koch for bacteria is _______.
Problem 4.3a
Immersion oil ________(increases/decreases) the numerical aperture, which ________(increases/decreases) resolution because _______(more/fewer) light rays are involved.
Problem 4.4a
________ refers to differences in intensity between two objects.
Problem 4.5a
Cationic chromophores such as methylene blue ionically bond to _______(positively/negatively) charged chemicals such as DNA and proteins.
Problem 5a
Which of the following factors is important in making an image appear larger?
a. thickness of the lens
b. curvature of the lens
c. speed of the light passing through the lens
d. all of the above
Problem 6a
Which of the following is different between light microscopy and transmission electron microscopy?
a. magnification
b. resolution
c. wavelengths
d. all of the above
Problem 7a
Which of the following types of microscopes produces a three- dimensional image with a shadowed appearance?
a. simple microscope
b. differential interference contrast microscope
c. fluorescence microscope
d. transmission electron microscope
Problem 8a
Which of the following microscopes combines the greatest magnification with the best resolution?
a. confocal microscope
b. phase-contrast microscope
c. dark-field microscope
d. bright-field microscope
Problem 9a
Negative stains such as eosin are also called _______.
a. capsule stains
b. endospore stains
c. simple stains
d. acid-fast stains
Problem 10
In the binomial system of nomenclature, which term is always written in lowercase letters?
a. Kingdom
b. Domain
c. Genus
d. Specific epithet
Ch. 4 - Microscopy, Staining, and Classification
