Immersion oil ________(increases/decreases) the numerical aperture, which ________(increases/decreases) resolution because _______(more/fewer) light rays are involved.

Verified step by step guidance

1. Understand that immersion oil is used in microscopy to improve image clarity.
2. Recognize that numerical aperture (NA) is a measure of a microscope objective's ability to gather light and resolve fine specimen detail at a fixed object distance.
3. Know that immersion oil increases the numerical aperture by reducing the refraction of light as it passes from the specimen into the objective lens.
4. Realize that an increased numerical aperture results in increased resolution, allowing finer details to be observed.
5. Conclude that more light rays are involved when immersion oil is used, enhancing the resolution of the image.

Key Concepts

Numerical Aperture

Numerical aperture (NA) is a dimensionless number that characterizes the range of angles over which a microscope objective can accept or emit light. It is a critical factor in determining the resolving power of a microscope. A higher NA indicates a greater ability to gather light and resolve fine specimen detail, which is essential for high-resolution imaging.

Resolution

Resolution refers to the ability of a microscope to distinguish between two closely spaced objects as separate entities. It is influenced by the numerical aperture and the wavelength of light used. Improved resolution allows for clearer and more detailed images, making it easier to observe fine structures within a specimen.

Immersion Oil

Immersion oil is used in microscopy to increase the numerical aperture of an objective lens. By placing oil between the lens and the specimen, it reduces light refraction and allows more light to enter the lens. This results in improved resolution, as more light rays contribute to the image formation, enhancing the clarity and detail of the observed specimen.