Four of the most abundant amino acids in proteins are leucine, alanine, glycine, and valine. What do these amino acids have in common? Would you expect these amino acids to be found on the interior or on the exterior of the protein?
As a chef, you prepare a wide variety of foods daily. The following dishes all contain protein. What method (if any) has been used to denature the protein present in each food?
a. Charcoal-grilled steak
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Key Concepts
Denaturation of Proteins
Cooking Methods and Heat Transfer
Maillard Reaction
For each amino acid listed, tell whether its influence on tertiary structure is largely through hydrophobic interactions, hydrogen bonding, formation of salt bridges, covalent bonding, or some combination of these effects.
a. Tyrosine
Fresh pineapple cannot be used in gelatin desserts because it contains an enzyme that hydrolyzes the proteins in gelatin, destroying the gelling action. Canned pineapple can be added to gelatin with no problem. Why?
Oxytocin is a small peptide that is used to induce labor by causing contractions in uterine walls. It has the primary structure Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gln. This peptide is held in a cyclic configuration by a disulfide bridge. Draw a diagram of oxytocin, showing the disulfide bridge.
Explain how a protein is denatured by the following:
c. Organic solvents
Explain how a protein is denatured by the following:
b. Strong acids
