A DNA sequence encoding a five-amino acid polypeptide is given below.
...ACGGCAAGATCCCACCCTAATCAGACCGTACCATTCACCTCCT...
...TGCCGTTCTAGGGTGGGATTAGTCTGGCATGGTAAGTGGAGGA...
Assuming the sequence above is a bacterial gene, identify the region encoding the Shine–Dalgarno sequence.

Question

A DNA sequence encoding a five-amino acid polypeptide is given below.

...ACGGCAAGATCCCACCCTAATCAGACCGTACCATTCACCTCCT...

...TGCCGTTCTAGGGTGGGATTAGTCTGGCATGGTAAGTGGAGGA...

Assuming the sequence above is a bacterial gene, identify the region encoding the Shine–Dalgarno sequence.

Accepted Answer

Hi, everyone. Welcome back. Here's our next question. The Shine Del Garno sequence is a ribosome binding site in bacterial and ARL messenger RN A generally located around eight pieces, eight bases upstream of the start code on A UG. The RN A sequence helps recruit the ribosome to the messenger RN am RN A to initiate protein synthesis by aligning the ribosome with a start codon. The conservation of a similar sequence between distantly related U carry outs imply that this nucleotide tract played an important role in the cell. What is the role proposed by this sequence in U Riots in protein synthesis, choice, a initiation of translation, choice B, elongation of translation, choice, C, termination of translation or choice D, none of these. So we're talking about the at first, the Shine Del Garno sequence, which is this ribosome binding site and it's binds the small. So the SV sequence binds the small subunit of RRN A near its three prime end in pro carry ups where as our question says, it's involved in the initiation of translation. But our question is saying there is another conserved sequence in you Caros. And our question doesn't tell us but it's also uh binds the small subunit of RRN A near the three prime end. But it's a slightly different consensus sequence like the Shine Del Garno sequence, it's highly conserved across very different species and new carats. And our question is saying, what is the role that's proposed? Again, knowing it's a really important role preserved across all these different new carats and the interest choice c termination of translation in the conservation sequence or consensus sequence. Excuse me, it's a complimentary to all three stop codons. And so that kind of provides us the mechanism of how it works to become involved in the termination of translation since it does have that recognition, that complementarity to the stop Coonts. So it's different from that in pro carats choice, a initiation of translation is not our answer as is the purpose of the Shine Del Garno sequence and not in the elongation. And then of course, choice D none of these is not correct since I do have an answer. But again, the role that is proposed by this consensus sequences in you Caros is choice C termination of translation. See you in the next video.

A DNA sequence encoding a five-amino acid polypeptide is given below.
...ACGGCAAGATCCCACCCTAATCAGACCGTACCATTCACCTCCT...
...TGCCGTTCTAGGGTGGGATTAGTCTGGCATGGTAAGTGGAGGA...
Assuming the sequence above is a bacterial gene, identify the region encoding the Shine–Dalgarno sequence.

Verified video answer for a similar problem:

Key Concepts

Shine-Dalgarno Sequence

Translation Initiation

Bacterial Gene Structure