Do the amino acids that are in the active site of an enzyme have to be near each other in the enzyme’s primary structure? If no, explain.

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Hello, everyone. Our next prop says which of the statements are true about the interaction of amino acids in the active site of an enzyme's primary structure. And we have four statements and then four answer choices. A one and three B three and four, C 13 and four or D 123 and four. So let's make our way through to these statements. Number one says the primary structure of an enzyme directly determines the specific sequence of amino acids in the active site influencing substrate binding. Well, this one is correct. That's the meaning of the primary sequence of a protein is that you know, specific order of amino acids. And specifically when we talk about the active site, those amino acids that line the active site of the enzyme and interactions between these amino acids and the substrate are what determines binding. So, since number one is a true statement, we will go ahead and cross out choice B three and four and that's it. Lemon. Two says amino acids in the primary structure can influence the enzyme's overall three dimensional shape, but they do not directly affect the active sites properties. Well, this is going to be an incorrect statement. Yes, amino acids are, you know, the nature of the primary structure of the protein. But the active site, if you remember has to bind to the substrate. But again, it's not just in shape, it's also in how it might interact with a substrate if the amino acids in that pocket are, you know, charged versus non polar, for instance. So if number two is incorrect, we can cross out choice D 123 and four. Now let's look at statement number three says interactions between amino acids in the active site are essential for the enzyme's specificity and catalytic ability. And yes, that is key. That is how the enzyme manages to be so specific and catalyze these reactions is this mm way that the amino acids in the active site interact specifically with the substrate. And then finally, statement number four, changes in the primary structure of an enzyme can lead to alterations in the active sites configuration and enzyme activity. If the enzyme, you know changes residues that interact with the substrate in a certain way, then it can't perform its proper job. So statement four is also correct. So our answer here will be choice C 13 and four. Well, we just eliminate choice A one and three. So once again, the true statements about the interaction of amino acids in the active site of an enzyme's primary structure is choice C 13 and four. See you in the next video.

Do the amino acids that are in the active site of an enzyme have to be near each other in the enzyme’s primary structure? If no, explain.

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Key Concepts

Enzyme Structure

Active Site

Protein Folding