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Ch. 21 - Genomic Analysis
Klug - Concepts of Genetics  12th Edition
Klug12th EditionConcepts of Genetics ISBN: 9780135564776Not the one you use?Change textbook
Chapter 21, Problem 20a

An interactive Web site for the Human Proteome Map (HPM) is available at http://www.humanproteomemap.org. Visit this site, and then answer the question.
How many proteins were identified in this project?

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1
Understand that the Human Proteome Map (HPM) project aimed to identify and catalog proteins expressed in various human tissues using mass spectrometry and other proteomic techniques.
Visit the official Human Proteome Map website at http:\/\/www.humanproteomemap.org to access the data and resources provided by the project.
Look for sections such as 'About', 'Data', or 'Summary' on the website, where the total number of proteins identified is typically reported.
Identify the reported total number of proteins detected across all tissues and samples analyzed in the project. This number represents the proteins experimentally confirmed by the HPM.
Note down the number of proteins as stated on the website, which answers the question about how many proteins were identified in the Human Proteome Map project.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Human Proteome Map (HPM)

The Human Proteome Map is a comprehensive resource that catalogs proteins expressed in various human tissues and organs. It integrates mass spectrometry data to identify and quantify proteins, providing insights into protein expression patterns across the human body.
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Proteomics and Protein Identification

Proteomics is the large-scale study of proteins, focusing on their structures and functions. Protein identification typically involves mass spectrometry techniques that detect peptides, which are then matched to known protein sequences to determine which proteins are present in a sample.
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Data Interpretation from Online Biological Databases

Interpreting data from biological databases like the HPM requires understanding how to navigate the site, locate summary statistics, and interpret protein counts. This skill is essential for extracting specific information such as the total number of proteins identified in a project.
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Bioinformatics
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Metagenomics studies generate very large amounts of sequence data. Provide examples of genetic insight that can be learned from metagenomics.

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Textbook Question

What are DNA microarrays? How are they used?

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Textbook Question

Annotation of the human genome sequence reveals a discrepancy between the number of protein-coding genes and the number of predicted proteins actually expressed by the genome. Proteomic analysis indicates that human cells are capable of synthesizing more than 100,000 different proteins and perhaps three times this number. What is the discrepancy, and how can it be reconciled?

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Textbook Question

An interactive Web site for the Human Proteome Map (HPM) is available at http://www.humanproteomemap.org. Visit this site, and then answer the question.

How many fetal tissues were analyzed?

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Textbook Question

An interactive Web site for the Human Proteome Map (HPM) is available at http://www.humanproteomemap.org. Visit this site, and then answer the question.

Use the 'Query' tab and select the 'Gene family' dropdown menu to do a search on the distribution of proteins encoded by a pathway of interest to you. Search in fetal tissues, adult tissues, or both.

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Textbook Question

Researchers have compared candidate loci in humans and rats in search of loci in the human genome that are likely to contribute to the constellation of factors leading to hypertension [Stoll, M., et al. (2000). Genome Res. 10:473–482]. Through this research, they identified 26 chromosomal regions that they consider likely to contain hypertension genes. How can comparative genomics aid in the identification of genes responsible for such a complex human disease? The researchers state that comparisons of rat and human candidate loci to those in the mouse may help validate their studies. Why might this be so?

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