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Ch. 5 - Genetic Linkage and Mapping in Eukaryotes
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 5, Problem 23c

A wild-type trihybrid soybean plant is crossed to a pure-breeding soybean plant with the recessive phenotypes pale leaf (l), oval seed (r), and short height (t). The results of the three-point test cross are shown below. Traits not listed are wild type.
Table displaying phenotypes and their corresponding numbers from a trihybrid soybean plant cross.
Calculate the interference value for these data.

Verified step by step guidance
1
Step 1: Identify the parental phenotypes and the double crossover phenotypes. The parental phenotypes are the most frequent classes (Pale and Oval, Short), while the double crossover phenotypes are the least frequent classes (Oval and Pale, Oval, Short).
Step 2: Determine the gene order by comparing the parental and double crossover phenotypes. The gene that differs between the parental and double crossover phenotypes is the middle gene.
Step 3: Calculate the recombination frequencies for the two intervals. Use the formula: Recombination frequency = (Number of recombinant offspring in the interval / Total offspring) × 100. Include single and double crossover offspring for each interval.
Step 4: Calculate the expected number of double crossovers using the product of the recombination frequencies for the two intervals (expressed as decimals) multiplied by the total number of offspring.
Step 5: Calculate the interference value using the formula: Interference = 1 - (Observed double crossovers / Expected double crossovers). Use the observed number of double crossovers from the data and the expected number calculated in Step 4.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Trihybrid Cross

A trihybrid cross involves three pairs of contrasting traits, allowing the study of inheritance patterns for multiple genes simultaneously. In this case, the traits are leaf color, seed shape, and plant height. Understanding how these traits segregate and assort independently is crucial for analyzing the offspring's phenotypes and genotypes.
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Three-Point Test Cross

A three-point test cross is a genetic cross used to determine the arrangement of genes on a chromosome and the distances between them. By crossing a trihybrid organism with a homozygous recessive organism, researchers can observe the offspring's phenotypes to infer gene linkage and recombination frequencies, which are essential for calculating genetic interference.
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Genetic Interference

Genetic interference refers to the phenomenon where the occurrence of one crossover event in a region of a chromosome affects the likelihood of another crossover occurring nearby. It is quantified using the interference value, which can be calculated from the observed and expected double crossover frequencies. Understanding interference helps in mapping genes and understanding genetic linkage.
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Related Practice
Textbook Question

T. H. Morgan's data on eye color and wing form genetic linkage between the two genes. Test the genetic linkage (shown in the figure below) data with chi-square analysis, and show that the results are significantly different from the expectation under the assumption of independent assortment.

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Textbook Question

A wild-type trihybrid soybean plant is crossed to a pure-breeding soybean plant with the recessive phenotypes pale leaf (l), oval seed (r), and short height (t). The results of the three-point test cross are shown below. Traits not listed are wild type.

What are the alleles on each homologous chromosome of the parental wild-type trihybrid soybean plant? Place the alleles in their correct gene order. Use L, R, and T to represent dominant alleles and l, r, and t for recessive alleles.

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Textbook Question

A wild-type trihybrid soybean plant is crossed to a pure-breeding soybean plant with the recessive phenotypes pale leaf (l), oval seed (r), and short height (t). The results of the three-point test cross are shown below. Traits not listed are wild type.

Calculate the recombination frequencies between the adjacent genes.

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Textbook Question

The boss in your laboratory has just heard of a proposal by another laboratory that genes for eye color and the length of body bristles may be linked in Drosophila. Your lab has numerous pure-breeding stocks of Drosophila that could be used to verify or refute genetic linkage. In Drosophila, red eyes (c⁺) are dominant to brown eyes (c) and long bristles (d⁺) are dominant to short bristles (d). Your lab boss asks you to design an experiment to test the genetic linkage of eye color and bristle-length genes, and to begin by crossing a pure-breeding line homozygous for red eyes and short bristles to a pure-breeding line that has brown eyes and long bristles.


Give the genotypes of the pure-breeding parental flies and the genotype(s) and phenotype(s) of the F₁ progeny they produce.

380
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Textbook Question

The boss in your laboratory has just heard of a proposal by another laboratory that genes for eye color and the length of body bristles may be linked in Drosophila. Your lab has numerous pure-breeding stocks of Drosophila that could be used to verify or refute genetic linkage. In Drosophila, red eyes (c⁺) are dominant to brown eyes (c) and long bristles (d⁺) are dominant to short bristles (d). Your lab boss asks you to design an experiment to test the genetic linkage of eye color and bristle-length genes, and to begin by crossing a pure-breeding line homozygous for red eyes and short bristles to a pure-breeding line that has brown eyes and long bristles.


In your experimental design, what are the genotype and phenotype of the line you propose to cross to the F₁ to obtain the most useful information about genetic linkage between the eye color and bristle-length genes? Explain why you make this choice.

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Textbook Question

The boss in your laboratory has just heard of a proposal by another laboratory that genes for eye color and the length of body bristles may be linked in Drosophila. Your lab has numerous pure-breeding stocks of Drosophila that could be used to verify or refute genetic linkage. In Drosophila, red eyes (c⁺) are dominant to brown eyes (c) and long bristles (d⁺) are dominant to short bristles (d). Your lab boss asks you to design an experiment to test the genetic linkage of eye color and bristle-length genes, and to begin by crossing a pure-breeding line homozygous for red eyes and short bristles to a pure-breeding line that has brown eyes and long bristles.


Assume the eye color and bristle-length genes are separated by 28 m.u. What are the approximate frequencies of phenotypes expected from the cross you proposed in part (b)?

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