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Ch. 9 - The Molecular Biology of Translation
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 9, Problem 39l

Answer the following questions about the accompanying diagram.
Diagram illustrating the process of translation, showing molecular components and interactions in a cell.
Does the diagram depict molecular activity in a bacterium or a eukaryote? Explain the reasoning for your answer. 

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Examine the diagram carefully. The image depicts a process involving DNA (labeled A), RNA (labeled C), and ribosomes (labeled D, E, F) synthesizing polypeptides (labeled G). This suggests the process of transcription and translation.
Identify the key features of the diagram. The RNA strand (C) is being synthesized from the DNA template (A) by the enzyme RNA polymerase (labeled B). Ribosomes (D, E, F) are attached to the RNA strand, actively translating it into polypeptides (G).
Consider the spatial arrangement of transcription and translation. In bacteria, transcription and translation occur simultaneously in the cytoplasm because there is no nuclear membrane separating these processes. In eukaryotes, transcription occurs in the nucleus, and translation occurs in the cytoplasm after RNA processing.
Note the absence of RNA processing steps such as splicing, capping, or polyadenylation in the diagram. These steps are characteristic of eukaryotic cells but are absent in bacterial cells.
Conclude based on the evidence. The simultaneous transcription and translation depicted in the diagram, along with the absence of RNA processing, strongly suggests that the molecular activity is occurring in a bacterium rather than a eukaryote.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Translation Process

Translation is the process by which ribosomes synthesize proteins using messenger RNA (mRNA) as a template. During translation, ribosomes read the sequence of codons in mRNA and assemble the corresponding amino acids into a polypeptide chain. This process is crucial for gene expression and occurs in both prokaryotic and eukaryotic cells, although the mechanisms and locations differ.
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Prokaryotic vs. Eukaryotic Cells

Prokaryotic cells, such as bacteria, lack a defined nucleus and membrane-bound organelles, while eukaryotic cells have a nucleus and various organelles. In prokaryotes, translation occurs in the cytoplasm simultaneously with transcription, whereas in eukaryotes, transcription occurs in the nucleus and translation occurs in the cytoplasm. This distinction is essential for determining the cellular context depicted in the diagram.
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Ribosomes and Their Structure

Ribosomes are complex molecular machines made of ribosomal RNA (rRNA) and proteins that facilitate the translation of mRNA into proteins. They consist of two subunits (large and small) that come together during translation. The presence and structure of ribosomes can indicate whether the diagram represents a prokaryotic or eukaryotic cell, as eukaryotic ribosomes are larger and more complex than those found in prokaryotes.
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