Laboratory Experiments in Microbiology, 12th edition
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Laboratory Experiments in Microbiology
ISBN-13: 9780134605203
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Overview
For general microbiology laboratory courses
Laboratory Experiments in Microbiology features 57 thoroughly class-tested and easily customizable exercises that teach basic microbiology techniques and applications. The manual provides comprehensive coverage of every area of microbiology across diverse disciplines, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The lab manual is the perfect companion to Tortora/Funke/Case’s Microbiology: An Introduction, 13th Edition or any introductory microbiology text.
The 12th Edition of Laboratory Experiments in Microbiology is easier than ever to navigate and more visually effective with new icons indicating when an exercise addresses the human or environmental microbiome, is investigative, or addresses an ASM guideline. New ASM Thinking Skills outline the steps that help develop laboratory thinking skills. Pre-lab quizzes in Mastering™ Microbiology ensure students arrive prepared for each lab, and activities such as Lab and Lecture: Putting It All Together help students see how lab and lecture are integrated.
Table of contents
Part One Microscopy
1. Use and Care of the Microscope
2. Examination of Living Microorganisms
Part Two Handling Bacteria
3. Microbes in the Environment
4. Transfer of Bacteria: Aseptic Technique
Part Three Staining Methods
5. Preparation of Smears and Simple Staining
6. Negative Staining
7. Gram Staining
8. Acid-Fast Staining
9. Structural Stains (Endospore, Capsule, and Flagella)
10. Morphological Unknown
Part Four Cultivation of Bacteria
11. Isolation of Bacteria by Dilution Techniques
12. Special Media for Isolating Bacteria
Part Five Microbial Metabolism
13. Carbohydrate Catabolism
14. Fermentation
15. Protein Catabolism, Part 1
16. Protein Catabolism, Part 2
17. Respiration
18. Unknown Identification and Bergey’s Manual
Part Six Microbial Growth
19. Oxygen and the Growth of Bacteria
20. Determination of a Bacterial Growth Curve: The Role of Temperature
21. Biofilms
Part Seven Control of Microbial Growth
22. Physical Methods of Control: Heat
23. Physical Methods of Control: Ultraviolet Radiation
24. Chemical Methods of Control: Disinfectants and Antiseptics
25. Chemical Methods of Control: Antimicrobial Drugs
26. Effectiveness of Hand Scrubbing
Part Eight Microbial Genetics
27. Regulation of Gene Expression
28. Isolation of Bacterial Mutants
29. Transformation of Bacteria
30. DNA Fingerprinting
31. Genetic Engineering
32. Ames Test for Detecting Possible Chemical Carcinogens
Part Nine The Microbial World
33. Fungi: Yeasts and Molds
34. Phototrophs: Algae and Cyanobacteria
35. Protozoa
36. Parasitic Helminths
Part Ten Viruses
37. Isolation and Titration of Bacteriophages
38. Plant Viruses
Part Eleven Interaction of Microbe and Host
39. Epidemiology
40. Koch's Postulate
Part Twelve Immunology
41. Innate Immunity
42. Agglutination Reactions: Slide Agglutination
43. Agglutination Reactions: Microtiter Agglutination
44. ELISA Technique
Part Thirteen Microrganisms and Disease
45. Bacteria of the Skin
46. Bacteria of the Respiratory Tract
47. Bacteria of the Mouth
48. Bacteria of the Gastrointestinal Tract
49. Bacteria of the Urogenital Trac
50. Identification of an Unknown from a Clinical Sample
51. Rapid Identification Methods
Part Fourteen Microbiology and the Environment
52. Microbes in Water: Multiple-Tube Technique
53. Microbes in Water: Membrane Filter Technique
54. Microbes in Food: Contamination
55. Microbes Used in the Production of Foods
56. Microbes in Soil: The Nitrogen and Sulfur Cycles
57. Microbes in Soil: Bioremediaton
APPENDICES.
A. Pipetting
B. Dilution Techniques and Calculations
C. Use of the Spectrophotometer
D. Graphing
E. Use of the Dissecting Microscope
F. Use of the Membrane Filter
G. Electrophoresis
H. Keys to Bacteria
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