Textbook Question
Answer these questions concerning promoters.
Eukaryotic promoters are more variable than bacterial promoters. Explain why.
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Ch. 8 - Molecular Biology of Transcription and RNA Processing
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172
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Table of contents
- Ch. 1 - The Molecular Basis of Heredity, Variation, and Evolution64
- Ch. 2 - Transmission Genetics144
- Ch. 3 - Cell Division and Chromosome Heredity81
- Ch. 4 - Gene Interaction87
- Ch. 5 - Genetic Linkage and Mapping in Eukaryotes103
- Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages73
- Ch. 7 - DNA Structure and Replication71
- Ch. 8 - Molecular Biology of Transcription and RNA Processing79
- Ch. 9 - The Molecular Biology of Translation110
- Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization100
- Ch. 11 - Gene Mutation, DNA Repair, and Homologous Recombination86
- Ch. 12 - Regulation of Gene Expression in Bacteria and Bacteriophage90
- Ch. 13 - Regulation of Gene Expression in Eukaryotes38
- Ch. 14 - Analysis of Gene Function via Forward Genetics and Reverse Genetics72
- Ch. 15 - Recombinant DNA Technology and Its Applications69
- Ch. 16 - Genomics: Genetics from a Whole-Genome Perspective49
- Ch. 17 - Organelle Inheritance and the Evolution of Organelle Genomes27
- Ch. 18 - Developmental Genetics43
- Ch. 19 - Genetic Analysis of Quantitative Traits66
- Ch. 20 - Population Genetics and Evolution at the Population, Species, and Molecular Levels105
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
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Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA ProcessingProblem 4b
Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA ProcessingProblem 4bChapter 8, Problem 4b
The diagram below shows a DNA duplex. The template strand is identified, as is the location of the nucleotide. consensus sequences.
Assume this region contains a gene transcribed to form mRNA in a eukaryote. Identify the location of the most common promoter.
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Identify the template strand in the DNA duplex. The template strand is the strand that will be used by RNA polymerase to synthesize the complementary mRNA strand during transcription.
Understand that in eukaryotes, the promoter region is located upstream (toward the 5' end) of the transcription start site (TSS). The promoter contains specific consensus sequences that are recognized by transcription factors and RNA polymerase.
Locate the most common promoter consensus sequences in eukaryotes. These include the TATA box, typically found around 25-30 base pairs upstream of the TSS, and other regulatory elements such as the CAAT box (around -80) and GC-rich regions (variable positions).
Mark the positions of these consensus sequences relative to the TSS on the DNA duplex. For example, the TATA box is usually found at approximately -25, where the negative sign indicates upstream from the TSS.
Ensure that the identified promoter sequences are on the non-template strand (coding strand), as these sequences are recognized by transcription factors and RNA polymerase during the initiation of transcription.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
DNA Structure
DNA is composed of two strands forming a double helix, with each strand made up of nucleotides. Each nucleotide consists of a sugar, a phosphate group, and a nitrogenous base. Understanding the structure of DNA is crucial for identifying regions such as the template strand and the location of genes, which are essential for transcription.
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06:25
DNA Structure
Promoter Regions
Promoter regions are specific sequences of DNA located upstream of a gene that initiate transcription. In eukaryotes, these regions often contain consensus sequences, such as the TATA box, which are recognized by transcription factors and RNA polymerase. Identifying these sequences is vital for understanding how genes are regulated and expressed.
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00:30Regions of X Chromosomes
Transcription in Eukaryotes
Transcription in eukaryotes involves the synthesis of mRNA from a DNA template. This process requires the binding of RNA polymerase to the promoter region, followed by the unwinding of the DNA and the addition of RNA nucleotides. Understanding this process is essential for identifying how genes are expressed and the role of various regulatory elements.
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Related Practice
Textbook Question
Answer these questions concerning promoters.
What is the meaning of the term alternative promoter? How does the use of alternative promoters affect transcription?
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Textbook Question
The diagram below shows a DNA duplex. The template strand is identified, as is the location of the nucleotide.
Assume this region contains a gene transcribed in a bacterium. Identify the location of promoter consensus sequences and of the transcription termination sequence.
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Textbook Question
The diagram below shows a DNA duplex. The template strand is identified, as is the location of the nucleotide.
If this region is a eukaryotic gene transcribed by RNA polymerase III, where are the promoter consensus sequences located?
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Textbook Question
The following is a portion of an mRNA sequence:
3'-AUCGUCAUGCAGA-5'
During transcription, was the adenine at the left-hand side of the sequence the first or the last nucleotide used to build the portion of mRNA shown? Explain how you know.
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Textbook Question
The following is a portion of an mRNA sequence:
3'-AUCGUCAUGCAGA-5'
Write out the sequence and polarity of the DNA duplex that encodes this mRNA segment. Label the template and coding DNA strands.
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