Robert Koch's postulate has four criterias including the following except:

The microorganism must be found in diseased but not in healthy individuals

The microorganism must be cultured from the diseased individual

Inoculation of a healthy individual with the cultured microorganism should recapitulate the disease

The microorganism must be re-isolated from the inoculated, diseased individual and matched to the original microorganism

None of the above