Question

In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92-26 °C, (b) 45-65 °C, and (c) 65-75 °C.

Accepted Answer

Hello everyone and welcome to today's video. So the binding of primers to the denature single stranded DNA A in PCR is called which of the following. Well, let's go over each of our answer choices so that we may solve the problem. First of all, we have DNA duration. So remember that D N A is going to be made up of two strands. So the nature in these trends means separating them, this is not going to be involving the binding of primers. So we're going to cancel this out. Then we have extension and extension is actually going to come after the binding of primers and it refers to the addition of nuclear sites. So this is not going to be the correct answer choice either and we're going to cancel it out. Then we have amplification. Well, amplification refers to the whole process. Amplification refers to making many copies of D N A which in this case is what PCR is going to be doing overall. So none of these answer choices belong to what the question is asking for. However, a kneeling is going to be our correct answer choice because another word for binding is actually going to be a kneeling and when we are unveiling during PCR, we're actually going to be binding primers to are they already the nature single stranded DNA? So because of this, this is going to be or correct answer choice. I really hope this video helped you and I hope to see you on the next one.

In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92-26 °C, (b) 45-65 °C, and (c) 65-75 °C.

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