Why were ³²P and ³⁵S chosen for use in the Hershey–Chase experiment? Discuss the rationale and conclusions of this experiment.
Ch. 10 - DNA Structure and Analysis

Chapter 10, Problem 9
What are the exceptions to the general rule that DNA is the genetic material in all organisms? What evidence supports these exceptions?
Verified step by step guidance1
Step 1: Understand the general rule that DNA is the genetic material in most organisms, meaning DNA carries the genetic information necessary for inheritance and cellular function.
Step 2: Identify the main exceptions to this rule, which are certain viruses that use RNA instead of DNA as their genetic material. Examples include retroviruses like HIV and some single-stranded RNA viruses.
Step 3: Explain that in these RNA viruses, RNA carries the genetic information and directs the synthesis of viral proteins, effectively functioning as the genetic material.
Step 4: Discuss the key experimental evidence supporting these exceptions, such as the Hershey-Chase experiment that confirmed DNA as genetic material in most organisms, and the discovery of RNA viruses where RNA was shown to be infectious and capable of replication.
Step 5: Highlight additional evidence from experiments with retroviruses, where reverse transcriptase enzyme converts viral RNA into DNA inside host cells, demonstrating RNA's role as genetic material in these cases.

Verified video answer for a similar problem:
This video solution was recommended by our tutors as helpful for the problem above.
Video duration:
3mWas this helpful?
Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
DNA as the Genetic Material
DNA is the molecule that stores genetic information in most organisms, directing development and cellular functions. This concept is foundational in genetics, as DNA's structure and replication explain heredity. The general rule states that DNA carries the genetic code in all living cells.
Recommended video:
Guided course
History and Experiments
RNA as Genetic Material in Some Viruses
Certain viruses use RNA instead of DNA to store genetic information, making RNA their genetic material. Examples include retroviruses like HIV and single-stranded RNA viruses like influenza. This exception challenges the universality of DNA as genetic material and highlights RNA's role in heredity.
Recommended video:
Guided course
History and Experiments
Experimental Evidence Supporting RNA as Genetic Material
Experiments such as the Hershey-Chase experiment and studies on RNA viruses demonstrated that RNA can carry genetic information. For instance, the Hershey-Chase experiment showed that DNA, not protein, is genetic material in bacteriophages, while other research confirmed RNA's role in viral replication, supporting exceptions to the DNA rule.
Recommended video:
Guided course
History and Experiments
Related Practice
Textbook Question
822
views
Textbook Question
Does the design of the Hershey–Chase experiment distinguish between DNA and RNA as the molecule serving as the genetic material? Why or why not?
990
views
Textbook Question
What observations are consistent with the conclusion that DNA serves as the genetic material in eukaryotes? List and discuss them.
819
views
Textbook Question
Draw the chemical structure of the three components of a nucleotide, and then link the three together. What atoms are removed from the structures when the linkages are formed?
856
views
Textbook Question
How are the carbon and nitrogen atoms of the sugars, purines, and pyrimidines numbered?
793
views
Textbook Question
Adenine may also be named 6-amino purine. How would you name the other four nitrogenous bases, using this alternative system? (O is indicated by 'oxy-,' and CH₃ by 'methyl.')
995
views
