Textbook Question
One strand of a fragment of duplex DNA has the sequence 5'-ATCGACCTGATC-3'.
What term is used to describe the pattern of base pairing between one DNA strand and its partner in a duplex?
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Ch. 7 - DNA Structure and Replication
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172
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Table of contents
- Ch. 1 - The Molecular Basis of Heredity, Variation, and Evolution64
- Ch. 2 - Transmission Genetics144
- Ch. 3 - Cell Division and Chromosome Heredity81
- Ch. 4 - Gene Interaction87
- Ch. 5 - Genetic Linkage and Mapping in Eukaryotes103
- Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages73
- Ch. 7 - DNA Structure and Replication71
- Ch. 8 - Molecular Biology of Transcription and RNA Processing79
- Ch. 9 - The Molecular Biology of Translation110
- Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization100
- Ch. 11 - Gene Mutation, DNA Repair, and Homologous Recombination86
- Ch. 12 - Regulation of Gene Expression in Bacteria and Bacteriophage90
- Ch. 13 - Regulation of Gene Expression in Eukaryotes38
- Ch. 14 - Analysis of Gene Function via Forward Genetics and Reverse Genetics72
- Ch. 15 - Recombinant DNA Technology and Its Applications69
- Ch. 16 - Genomics: Genetics from a Whole-Genome Perspective49
- Ch. 17 - Organelle Inheritance and the Evolution of Organelle Genomes27
- Ch. 18 - Developmental Genetics43
- Ch. 19 - Genetic Analysis of Quantitative Traits66
- Ch. 20 - Population Genetics and Evolution at the Population, Species, and Molecular Levels105
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 7, Problem 7
For the following fragment of DNA, determine the number of hydrogen bonds and the number of phosphodiester bonds present:
5'-ACGTAGAGTGCTC-3'
3'-TGCATCTCACGAG-5'
Verified step by step guidance1
Step 1: Understand the structure of DNA. DNA is composed of two strands that are complementary and antiparallel. Each base pair is connected by hydrogen bonds, and the backbone of each strand is connected by phosphodiester bonds.
Step 2: Count the hydrogen bonds between the base pairs. Adenine (A) pairs with Thymine (T) via 2 hydrogen bonds, and Cytosine (C) pairs with Guanine (G) via 3 hydrogen bonds. Analyze the sequence to determine the number of each base pair and calculate the total hydrogen bonds.
Step 3: Count the phosphodiester bonds in the DNA backbone. Phosphodiester bonds connect the sugar-phosphate backbone of each strand. For a single strand of DNA, the number of phosphodiester bonds is one less than the number of nucleotides. Count the nucleotides in one strand and subtract 1 to find the number of phosphodiester bonds in that strand.
Step 4: Multiply the phosphodiester bond count by 2 since there are two strands in the DNA molecule. This gives the total number of phosphodiester bonds in the double-stranded DNA.
Step 5: Combine the results from steps 2 and 4 to summarize the total number of hydrogen bonds and phosphodiester bonds present in the given DNA fragment.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Hydrogen Bonds
Hydrogen bonds are weak interactions that occur between complementary nitrogenous bases in DNA. In the double helix structure, adenine (A) pairs with thymine (T) through two hydrogen bonds, while cytosine (C) pairs with guanine (G) through three hydrogen bonds. Understanding the number of hydrogen bonds is crucial for determining the stability and structure of the DNA molecule.
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Phosphodiester Bonds
Phosphodiester bonds are covalent linkages that connect the sugar and phosphate groups of adjacent nucleotides in a DNA strand. These bonds form the backbone of the DNA molecule, providing structural integrity. Each DNA strand has a continuous chain of phosphodiester bonds, and recognizing their presence is essential for understanding the overall structure and function of DNA.
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Complementary Base Pairing
Complementary base pairing refers to the specific pairing of nitrogenous bases in DNA, where A pairs with T and C pairs with G. This principle is fundamental to DNA replication and transcription, as it ensures accurate copying of genetic information. Identifying the base pairs in the given DNA sequence is necessary to calculate the number of hydrogen bonds formed between the strands.
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Related Practice
Textbook Question
One strand of a fragment of duplex DNA has the sequence 5'-ATCGACCTGATC-3'.
What term is used to describe the polarity of two DNA strands in a duplex?
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Textbook Question
The principles of complementary base pairing and antiparallel polarity of nucleic acid strands in a duplex are universal for the formation of nucleic acid duplexes. What is the chemical basis for this universality?
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Textbook Question
The following figure (Figure 1.6) presents simplified depictions of nucleotides containing deoxyribose, a nucleotide base, and a phosphate group. Use this simplified method of representation to illustrate the sequence 3'-AGTCGAT-5' and its complementary partner in a DNA duplex.
What kind of bond joins the C to G within a single strand?
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Textbook Question
The following figure (Figure 1.6) presents simplified depictions of nucleotides containing deoxyribose, a nucleotide base, and a phosphate group. Use this simplified method of representation to illustrate the sequence 3'-AGTCGAT-5' and its complementary partner in a DNA duplex.
What kind of bonds join the C in one strand to the G in the complementary strand?
487
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Textbook Question
The following figure (Figure 1.6) presents simplified depictions of nucleotides containing deoxyribose, a nucleotide base, and a phosphate group. Use this simplified method of representation to illustrate the sequence 3'-AGTCGAT-5' and its complementary partner in a DNA duplex.
How many phosphodiester bonds are present in this DNA duplex?
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