Textbook Question
The following is a portion of an mRNA sequence:
3'-AUCGUCAUGCAGA-5'
Identify the direction in which the promoter region for this gene will be located.
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Ch. 8 - Molecular Biology of Transcription and RNA Processing
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172
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Table of contents
- Ch. 1 - The Molecular Basis of Heredity, Variation, and Evolution64
- Ch. 2 - Transmission Genetics144
- Ch. 3 - Cell Division and Chromosome Heredity81
- Ch. 4 - Gene Interaction87
- Ch. 5 - Genetic Linkage and Mapping in Eukaryotes103
- Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages73
- Ch. 7 - DNA Structure and Replication71
- Ch. 8 - Molecular Biology of Transcription and RNA Processing79
- Ch. 9 - The Molecular Biology of Translation110
- Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization100
- Ch. 11 - Gene Mutation, DNA Repair, and Homologous Recombination86
- Ch. 12 - Regulation of Gene Expression in Bacteria and Bacteriophage90
- Ch. 13 - Regulation of Gene Expression in Eukaryotes38
- Ch. 14 - Analysis of Gene Function via Forward Genetics and Reverse Genetics72
- Ch. 15 - Recombinant DNA Technology and Its Applications69
- Ch. 16 - Genomics: Genetics from a Whole-Genome Perspective49
- Ch. 17 - Organelle Inheritance and the Evolution of Organelle Genomes27
- Ch. 18 - Developmental Genetics43
- Ch. 19 - Genetic Analysis of Quantitative Traits66
- Ch. 20 - Population Genetics and Evolution at the Population, Species, and Molecular Levels105
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
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Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA ProcessingProblem 7b
Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA ProcessingProblem 7bChapter 8, Problem 7b
The DNA sequences shown below are from the promoter regions of six bacterial genes. In each case, the last nucleotide in the sequence (highlighted in blue) is the nucleotide that initiates transcription. Determine the consensus sequence for the Pribnow box from these sequences.
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Identify the Pribnow box region in each sequence. The Pribnow box is typically located approximately 10 base pairs upstream of the transcription start site (highlighted in blue). Extract the corresponding region from each sequence.
Align the extracted Pribnow box regions from all six sequences. Ensure that the sequences are properly aligned by their relative positions to the transcription start site.
For each position in the aligned sequences, count the frequency of each nucleotide (A, T, G, C). This will help determine the most common nucleotide at each position.
Determine the consensus sequence by selecting the nucleotide with the highest frequency at each position in the Pribnow box alignment. If there is a tie, note that ambiguity may exist at that position.
Combine the most frequent nucleotides from each position to form the final consensus sequence for the Pribnow box.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Pribnow Box
The Pribnow box, also known as the -10 region, is a conserved sequence found in bacterial promoters, typically located about 10 nucleotides upstream of the transcription start site. It plays a crucial role in the initiation of transcription by providing a binding site for RNA polymerase. The consensus sequence for the Pribnow box is usually represented as 'TATAAT', which is essential for the recognition and binding of the transcription machinery.
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Prokaryotic Transcription
Consensus Sequence
A consensus sequence is a sequence of DNA that represents the most common nucleotides found at each position in a set of related sequences. It is derived from multiple sequences and highlights the nucleotides that are most frequently observed, indicating their importance in biological functions. In the context of the Pribnow box, the consensus sequence helps identify the key elements necessary for transcription initiation in bacteria.
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08:55Sequencing Overview
Transcription Initiation
Transcription initiation is the process by which RNA polymerase binds to a promoter region of DNA and begins synthesizing RNA. This process is critical for gene expression, as it determines when and how much of a gene is transcribed into messenger RNA. The last nucleotide in the promoter sequence, highlighted in the question, marks the precise point where transcription starts, making it essential for understanding the regulation of gene expression in bacteria.
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Related Practice
Textbook Question
Compare and contrast the properties of DNA polymerase and RNA polymerase, listing at least three similarities and at least three differences between the molecules.
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Textbook Question
The DNA sequences shown below are from the promoter regions of six bacterial genes. In each case, the last nucleotide in the sequence (highlighted in blue) is the nucleotide that initiates transcription. Examine these sequences and identify the Pribnow box sequence at approximately -10 for each promoter.
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Textbook Question
Bacterial and eukaryotic gene transcripts can differ—in the transcripts themselves, in whether the transcripts are modified before translation, and in how the transcripts are modified. For each of these three areas of contrast, describe what the differences are and why the differences exist.
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Textbook Question
Describe the two types of transcription termination found in bacterial genes. How does transcription termination differ for eukaryotic genes?
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Textbook Question
What is the role of enhancer sequences in transcription of eukaryotic genes? Speculate about why enhancers are not part of transcription of bacterial genes.
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