Textbook Question
The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.
Label the introns.
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Ch. 8 - Molecular Biology of Transcription and RNA Processing
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172
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Table of contents
- Ch. 1 - The Molecular Basis of Heredity, Variation, and Evolution64
- Ch. 2 - Transmission Genetics144
- Ch. 3 - Cell Division and Chromosome Heredity81
- Ch. 4 - Gene Interaction87
- Ch. 5 - Genetic Linkage and Mapping in Eukaryotes103
- Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages73
- Ch. 7 - DNA Structure and Replication71
- Ch. 8 - Molecular Biology of Transcription and RNA Processing79
- Ch. 9 - The Molecular Biology of Translation110
- Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization100
- Ch. 11 - Gene Mutation, DNA Repair, and Homologous Recombination86
- Ch. 12 - Regulation of Gene Expression in Bacteria and Bacteriophage90
- Ch. 13 - Regulation of Gene Expression in Eukaryotes38
- Ch. 14 - Analysis of Gene Function via Forward Genetics and Reverse Genetics72
- Ch. 15 - Recombinant DNA Technology and Its Applications69
- Ch. 16 - Genomics: Genetics from a Whole-Genome Perspective49
- Ch. 17 - Organelle Inheritance and the Evolution of Organelle Genomes27
- Ch. 18 - Developmental Genetics43
- Ch. 19 - Genetic Analysis of Quantitative Traits66
- Ch. 20 - Population Genetics and Evolution at the Population, Species, and Molecular Levels105
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
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Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA ProcessingProblem 16b
Sanders 3rd EditionCh. 8 - Molecular Biology of Transcription and RNA ProcessingProblem 16bChapter 8, Problem 16b
The segment of the bacterial TrpA gene involved in intrinsic termination of transcription is the following:
3'-TGGGTCGGGGCGGATTACTGCCCCGAAAAAAAACTTG-5'
5'-ACCCAGCCCCGCCTAATGACGGGGCTTTTTTTTGAAC-3'
Label the template and coding DNA strands.
Verified step by step guidance1
Determine the orientation of the DNA strands: The template strand is the one that RNA polymerase reads to synthesize RNA, while the coding strand has the same sequence as the RNA (except thymine is replaced with uracil in RNA).
Identify the 5' to 3' direction for each strand: The sequence provided shows the 3'-5' direction for the first strand and the 5'-3' direction for the second strand.
Label the template strand: The template strand is the one that is complementary to the RNA being synthesized. In this case, the 3'-TGGGTCGGGGCGGATTACTGCCCCGAAAAAAAACTTG-5' strand is the template strand.
Label the coding strand: The coding strand is the one that matches the RNA sequence (except for uracil replacing thymine). In this case, the 5'-ACCCAGCCCCGCCTAATGACGGGGCTTTTTTTTGAAC-3' strand is the coding strand.
Verify the labeling: Ensure that the template strand is complementary to the coding strand and that the coding strand matches the RNA sequence (with uracil replacing thymine).
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
DNA Strands
DNA consists of two strands: the template strand and the coding strand. The template strand is used by RNA polymerase to synthesize RNA during transcription, while the coding strand has the same sequence as the RNA (except for thymine being replaced by uracil). Understanding the roles of these strands is crucial for analyzing transcription processes.
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03:03
Double Strand Breaks
Transcription
Transcription is the process by which RNA is synthesized from a DNA template. It involves the enzyme RNA polymerase binding to the DNA at a promoter region, unwinding the DNA, and synthesizing a complementary RNA strand. Recognizing the significance of transcription is essential for understanding gene expression and regulation.
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09:16Eukaryotic Transcription
Intrinsic Termination
Intrinsic termination is a mechanism that signals the end of transcription in prokaryotes. It occurs when a specific sequence in the RNA forms a hairpin structure followed by a series of uracils, causing RNA polymerase to dissociate from the DNA. This concept is vital for understanding how transcription is precisely regulated and terminated.
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01:55Translation Termination
Related Practice
Textbook Question
The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.
Are intron regions single stranded or double stranded? Why?
659
views
Textbook Question
The segment of the bacterial TrpA gene involved in intrinsic termination of transcription is the following:
3'-TGGGTCGGGGCGGATTACTGCCCCGAAAAAAAACTTG-5'
5'-ACCCAGCCCCGCCTAATGACGGGGCTTTTTTTTGAAC-3' Draw the mRNA structure that forms during transcription of this segment of the TrpA gene.
546
views
Textbook Question
The segment of the bacterial TrpA gene involved in intrinsic termination of transcription is the following:
3'-TGGGTCGGGGCGGATTACTGCCCCGAAAAAAAACTTG-5'
5'-ACCCAGCCCCGCCTAATGACGGGGCTTTTTTTTGAAC-3'
Explain how a sequence of this type leads to intrinsic termination of transcription.
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Textbook Question
A 2-kb fragment of E. coli DNA contains the complete sequence of a gene for which transcription is terminated by the rho protein. The fragment contains the complete promoter sequence as well as the terminator region of the gene. The cloned fragment is examined by band shift assay. Each lane of a single electrophoresis gel contains the 2-kb cloned fragment under the following conditions:
Lane 1: 2-kb fragment alone
Lane 2: 2-kb fragment plus the core enzyme
Lane 3: 2-kb fragment plus the RNA polymerase holoenzyme
Lane 4: 2-kb fragment plus rho protein
Diagram the relative positions expected for the DNA fragments in this gel electrophoresis analysis.
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Textbook Question
A 2-kb fragment of E. coli DNA contains the complete sequence of a gene for which transcription is terminated by the rho protein. The fragment contains the complete promoter sequence as well as the terminator region of the gene. The cloned fragment is examined by band shift assay. Each lane of a single electrophoresis gel contains the 2-kb cloned fragment under the following conditions:
Lane 1: 2-kb fragment alone
Lane 2: 2-kb fragment plus the core enzyme
Lane 3: 2-kb fragment plus the RNA polymerase holoenzyme
Lane 4: 2-kb fragment plus rho protein
Explain the relative positions of bands in lanes 1 and 3.
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