What would be the sequence of the strand of DNA that is made from the following template: 5′-GATATCGAT-3′? (Your answer must be written 5'→3'.) How would the sequence be different if RNA were made from this DNA template?

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Hi everyone. Let's look at our next question, what would be the sequence of the strand of RNA that is made from the DNA template. And then it lists the sequence of DNA bases in the direction three prime five prime. So we need to kind of keep two things in mind here. One if we're drawing an RNA strand we have to remember that wherever there is an A. In the DNA sequence, we're gonna have to substitute you're a cell you for timing um where we'd have a. T. And D. N. A. As the compliment. And the other thing to remember our D. N. A. Temple has given to us in a three prime to five prime direction. This is going to be a complementary strand. So when we write our our Nystrand we'll want to make sure we note that it will be going from the five prime end to the three primate to make sure we note that because it can be written in both orders. And when we're comparing our answers to our choices we want to make sure we have that straight. So I've written five prime and then we're going to look at our D. N. A. Sequence and write the complementary bases remembering to use you instead of tea. So the first one here is a G. So we've got C. And then we've got an A. So that's gonna be you as the compliment in the RNA strand. And then T. So we write A. And a. So we write you and I'm gonna go through and write that out all the complementary bases. And then remembering the next other end will be the three prime end. So there's my complimentary RNA Strand. So now let's look over at my answer choices. And I'm just going to start by looking at the first three bases. Because if I were on a test in the interest of speed I might not need to match every single thing. I may be able to eliminate plenty of answers just looking at the first three here. So I'm gonna start that way and see what happens. So Choice A. Starts on the three prime end. So I have to remember to compare that and go in the other direction when I look at the sequence I wrote. So if I start on the three prime end, my first three bases are A. You see that doesn't match what's over here. So we eliminate choice A. Choice B. Starts on the five prime end my first three bases R. C. U. A. Which matches what I have. So choice B. Can state choice C. Starts on three prime. And I have the first three bases G. A. T. Which doesn't match my strand. So eliminate choice C. And finally Choice D. Starts on the five prime end. But it says C. Au where's my strand of see you a choice D. Also does not match. So just by looking at the first three bases I've found my correct answer here. Uh So on a test I would just pick that and move on just to be thorough. Here will make sure the rest of the strand matches. So I started with C. U. A. And I followed with you a G. C. U. A. So the entire strand matches in the proper direction compared to my RNA strand. So Choice B. Is my correct answer here. Thanks for watching. See you in the next video.

What would be the sequence of the strand of DNA that is made from the following template: 5′-GATATCGAT-3′? (Your answer must be written 5'→3'.) How would the sequence be different if RNA were made from this DNA template?

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Key Concepts

DNA Replication

Transcription

Nucleotide Base Pairing