Outline the roles of RNA in eukaryotic gene regulation.

Explain how a tissue-specific RNA-binding protein can lead to tissue-specific alternative splicing via splicing enhancers or splicing silencers.

The regulation of mRNA decay relies heavily upon deadenylases and decapping enzymes. Explain how these classes of enzymes are critical to initiating mRNA decay.

Nonsense-mediated decay is an mRNA surveillance pathway that eliminates mRNAs with premature stop codons. How does the cell distinguish between normal mRNAs and those with a premature stop?

What are processing bodies (P bodies), and what role do they play in mRNA regulation?

Explain how the addition of acetyl groups to histones leads to a weaker association of DNA in nucleosomes.

Compare and contrast promoters and enhancers with respect to their location (upstream versus downstream), orientation, and distance (in base pairs) relative to a gene they regulate.

Distinguish between the cis-acting regulatory elements referred to as promoters and enhancers.