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Ch. 3 - Mendelian Genetics
Klug - Concepts of Genetics  12th Edition
Klug12th EditionConcepts of Genetics ISBN: 9780135564776Not the one you use?Change textbook
Chapter 3, Problem 28a

To assess Mendel's law of segregation using tomatoes, a true-breeding tall variety (SS) is crossed with a true-breeding short variety (ss). The heterozygous F₁ tall plants (Ss) were crossed to produce two sets of F₂ data, as follows.
Table comparing two datasets showing counts of tall and short plants: Set I with 30 tall and 5 short, Set II with 300 tall and 50 short.
Using the X² test, analyze the results for both datasets. Calculate X² values and estimate the p values in both cases.

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Step 1: Determine the expected phenotypic ratio based on Mendel's law of segregation. Since the F₁ plants are heterozygous (Ss), the expected F₂ phenotypic ratio for tall (S_) to short (ss) plants is 3:1.
Step 2: Calculate the total number of plants in each set. For Set I, total = 30 tall + 5 short = 35 plants. For Set II, total = 300 tall + 50 short = 350 plants.
Step 3: Calculate the expected number of tall and short plants for each set using the 3:1 ratio. Expected tall = (3/4) × total, and expected short = (1/4) × total.
Step 4: Use the chi-square formula to calculate the chi-square (\$X^2\$) value for each set: \[ \chi^2 = \sum \frac{(\text{observed} - \text{expected})^2}{\text{expected}} \] Calculate this separately for tall and short plants and then sum the values.
Step 5: Determine the degrees of freedom (df) for the test, which is the number of phenotypic categories minus 1 (df = 2 - 1 = 1). Use a chi-square distribution table to estimate the p-value for each \$X^2\$ value to assess whether the observed data significantly deviate from the expected 3:1 ratio.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Mendel's Law of Segregation

Mendel's law of segregation states that allele pairs separate during gamete formation, so each gamete carries only one allele for each gene. In a monohybrid cross of heterozygotes (Ss x Ss), the expected phenotypic ratio is 3:1 (dominant to recessive). This principle explains the inheritance pattern observed in the tall and short tomato plants.
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Chi-Square (X²) Test

The chi-square test is a statistical method used to compare observed data with expected data to determine if deviations are due to chance. It calculates a value based on the sum of squared differences between observed and expected counts, divided by expected counts. This test helps assess if the observed tomato plant ratios fit Mendel's expected 3:1 ratio.
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p-Value and Statistical Significance

The p-value indicates the probability that the observed deviations from expected results occurred by chance. A low p-value (typically < 0.05) suggests significant deviation, rejecting the null hypothesis. In this context, it helps determine if the tomato plant data supports Mendel's law or if other factors might be influencing the results.
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Related Practice
Textbook Question

Datura stramonium (the Jimsonweed) expresses flower colors of purple and white and pod textures of smooth and spiny. The results of two crosses in which the parents were not necessarily true breeding are shown below. Assuming that true-breeding strains of all combinations of traits are available, what single cross could you execute and carry to an F₂ generation that will prove or disprove your hypothesis? Assuming your hypothesis is correct, what results of this cross will support it?

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Textbook Question

The wild-type (normal) fruit fly, Drosophila melanogaster, has straight wings and long bristles. Mutant strains have been isolated that have either curled wings or short bristles. The genes representing these two mutant traits are located on separate chromosomes. Carefully examine the data from the following five crosses.

Identify each mutation as either dominant or recessive. In each case, indicate which crosses support your answer.

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Textbook Question

The wild-type (normal) fruit fly, Drosophila melanogaster, has straight wings and long bristles. Mutant strains have been isolated that have either curled wings or short bristles. The genes representing these two mutant traits are located on separate chromosomes. Carefully examine the data from the following five crosses.

Assign gene symbols and, for each cross, determine the genotypes of the parents.

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Textbook Question

To assess Mendel's law of segregation using tomatoes, a true-breeding tall variety (SS) is crossed with a true-breeding short variety (ss). The heterozygous F₁ tall plants (Ss) were crossed to produce two sets of F₂ data, as follows.

From the above analysis, what can you conclude about the importance of generating large datasets in experimental conditions?

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Textbook Question

Albinism, caused by a mutational disruption in melanin (skin pigment) production, has been observed in many species, including humans. In 1991, and again recently in 2017, the only documented observations of an albino humpback whale (named 'Migaloo') were observed near New South Wales. Recently, Polanowski and coworkers (Polanowski, A., S. Robinson-Laverick, and D. Paton. (2012). Journal of Heredity 103:130–133) studied the genetics of humpback whales from the east coast of Australia, including Migaloo. Do you think that Migaloo's albinism is more likely caused by a dominant or recessive mutation? Explain your reasoning.

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Textbook Question

Albinism, caused by a mutational disruption in melanin (skin pigment) production, has been observed in many species, including humans. In 1991, and again recently in 2017, the only documented observations of an albino humpback whale (named 'Migaloo') were observed near New South Wales. Recently, Polanowski and coworkers (Polanowski, A., S. Robinson-Laverick, and D. Paton. (2012). Journal of Heredity 103:130–133) studied the genetics of humpback whales from the east coast of Australia, including Migaloo. What data would be helpful in determining the answer to part (a)?

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