How do we know that bacteriophages recombine genetic material through transduction and that cell-to-cell contact is not essential for transduction to occur?

Klug 12th Edition
Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages
Problem 2Write a short summary that contrasts how recombination occurs in bacteria and bacteriophages.
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Key Concepts
Bacterial Recombination Mechanisms
Phage Recombination Processes
Differences in Recombination Context and Purpose
In this chapter, we have focused on genetic systems present in bacteria and on the viruses that use bacteria as hosts (bacteriophages). In particular, we discussed mechanisms by which bacteria and their phages undergo genetic recombination, which allows geneticists to map bacterial and bacteriophage chromosomes. In the process, we found many opportunities to consider how this information was acquired. From the explanations given in the chapter, what answers would you propose to the following questions? How do we know that intergenic exchange occurs in bacteriophages?
In this chapter, we have focused on genetic systems present in bacteria and on the viruses that use bacteria as hosts (bacteriophages). In particular, we discussed mechanisms by which bacteria and their phages undergo genetic recombination, which allows geneticists to map bacterial and bacteriophage chromosomes. In the process, we found many opportunities to consider how this information was acquired. From the explanations given in the chapter, what answers would you propose to the following questions? How do we know that in bacteriophage T4 the rII locus is subdivided into two regions, or cistrons?
With respect to F⁺ and F⁻ bacterial matings, answer the following questions: How was it established that physical contact between cells was necessary?
With respect to F⁺ and F⁻ bacterial matings, answer the following questions: How was it established that chromosome transfer was unidirectional?
With respect to F⁺ and F⁻ bacterial matings, answer the following questions: What is the genetic basis for a bacterium's being F⁺.