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  5. Biochemistry Laboratory: Modern Theory and Techniques

Overview

Your biochemistry lab course is an essential component in training for a career in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Biochemistry Laboratory: Modern Theory and Techniques covers the theories, techniques, and methodologies practiced in the biochemistry teaching and research lab. Instead of specific experiments, it focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail.  An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.

Table of contents

Chapter 1: Introduction to the Biochemistry/Molecular Biology Laboratory
Chapter 2: Using the Computer and Internet for Research in Biochemistry
Chapter 3: General Laboratory Procedures
Chapter 4: Centrifugation Techniques in Biochemistry/Molecular Biology
Chapter 5: Purification and Analysis of Biomolecules by Chromatography
Chapter 6: Characterization of Proteins and Nucleic Acids by Electrophoresis
Chapter 7: Spectroscopic Analysis of Biomolecules
Chapter 8: Biomolecular Interactions: Ligand Binding and Enzyme Reacations
Chapter 9: Molecular Biology I: Structures and Analysis of Nucleic Acids
Chapter 10: Molecular Biology II: Recombinant DNA, Molecular Cloning and Enzymology
Chapter 11: Protein Production, Purification, and Characterization

Appendices
Index

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