Basic Laboratory Methods for Biotechnology, 2nd Edition
©2009 |Pearson | Available
Lisa A. Seidman, Madison Area Technical College
Cynthia J. Moore, Illinois State University
©2009 |Pearson | Available
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For laboratory courses in Biotechnology.
Presented from the perspective of the biotech industry, this laboratory handbook/textbook reference gives a systematic, understandable, and practical introduction to fundamental laboratory methods and provides a foundation upon which students can build a career in the lab. The authors balance background and theory with practical information, drawing material from many sources: analytical chemistry texts, molecular biology manuals, industry standards, government regulations, manufacturer and supplier information, and the useful laboratory “lore” that is part of the industry’s oral tradition.
The Second Edition features two new chapters covering Quality Assays and Tests (Chapters 24 and 25) and a new chapter on Culture Media for Intact Cells (Chapter 30). The authors have made revisions to update the safety chapters, the methods chapters, and have reorganized the units so that the information is presented in an order that mimics a typical class. They have also completely rewritten the Computers and Biotechnology chapters as well as the introductory chapter to more accurately reflect the evolving nature of the biotechnology industry. This new edition also reflects the changes in the field of biotechnology, how profit and other metrics affect research, the pursuit of new ideas, and the implementation of product testing and government approval.
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1. The Modern Biotechnology Industry: A Broad Overview 2. The Business of Biotechnology: The Transformation of Knowledge into Products 3. Pharmaceutical/Biopharmaceutical Products II. PRODUCT QUALITY AND BIOTECHNOLOGY 4. Introduction to Product Quality Systems 5. Biotechnology and the Regulation of Food and Medical Products 6. Documentation, the Foundation of Quality 7. Quality Systems in the Production Facility 8. Quality Systems in the Laboratory III. SAFETY IN THE LABORATORY 9. Introduction to a Safe Workplace 10. Working Safely in the Laboratory: General Considerations and Physical Hazards 11. Working Safely with Chemicals 12. Working Safely with Biological Materials IV. MATH IN THE BIOTECHNOLOGY LABORATORY: AN OVERVIEW 13. Basic Math Techniques 14. Proportional Relationships 15. Relationships and Graphing 16. Descriptions of Data (Descriptive Statistics) V. LABORATORY MEASUREMENTS 17. Introduction to Quality Laboratory Measurements, Tests and Assays 18. Introduction to Instrumental Methods and Electricity 19. The Measurement of Weight 20. The Measurement of Volume 21. The Measurement of Temperature 22. The Measurement of pH, Selected Ions and Conductivity 23. Measurements Involving Light A. Basic Principles and Instrumentation VI. QUALITY ASSAYS AND TESTS 24. Introduction to Quality Laboratory Tests and Assays 25. Measurements Involving Light B. Applications and Methods VII. LABORATORY SOLUTIONS 26. Preparation of Laboratory Solutions A: Concentration Expressions and Calculations 27. Preparation of Laboratory Solutions B. Basic Procedures and Practical Information 28. Solutions: Associated Procedures and Information 29. Laboratory Solutions to Support the Activity of Biological Macromolecules 30. Culture Media for Intact Cells VII. BASIC SEPARATION METHODS 30. Introduction to Filtration 31. Introduction to Centrifugation 32. Introduction to Bioseparations IX. COMPUTERS IN THE LABORATORY 33. Computers: An Overview 34: Data Handling with Computers 35. Applications of the Internet to Biotechnology
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Seidman & Moore
©2009  | Pearson  | 800 pp
Lisa Seidman received her Ph.D. from the University of Wisconsin and has taught for more than 20 years in the Biotechnology Laboratory Technician Program at Madison Area Technical College in Madison, Wisconsin. Cynthia Moore received her Ph.D. in Microbiology from Temple University School of Medicine. She is currently an Associate Professor of Biological Sciences at Illinois State University, where she also serves as Director of Biology Teacher Education.
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