Which of the following is not one of the three major conditions of enzymes?

• A. Enzymes cannot be consumed by a reaction
• B. Enzymes cannot be changed by a reaction
• C. Enzymes do not effect the rate of the reaction, only the free energy
• D. Enzymes do not effect the free energy of the reaction, only the reaction rate

Answer: (C)

Verified step by step guidance

1. Understand the role of enzymes: Enzymes are biological catalysts that speed up chemical reactions without being consumed or permanently altered in the process.
2. Identify the three major conditions of enzymes: Enzymes are not consumed by the reaction, they are not changed by the reaction, and they do not affect the free energy of the reaction.
3. Clarify the concept of free energy: Enzymes lower the activation energy required for a reaction, which increases the reaction rate, but they do not change the overall free energy change (ΔG) of the reaction.
4. Evaluate the given statements: The statement 'Enzymes do not affect the rate of the reaction, only the free energy' is incorrect because enzymes do affect the reaction rate by lowering the activation energy.
5. Conclude which statement is not a major condition: The incorrect statement about enzymes not affecting the reaction rate is the one that does not align with the fundamental properties of enzymes.